Senior Research Scientist

Senior Research Scientist

The University of Pittsburgh's School of Medicine, Department of Internal Medicine, Division of Rheumatology and Clinical Immunology seeks a Senior Research Scientist in Pittsburgh, PA.

Duties include:

(i) independently leading scientific research projects and supervising trainees and research activities; (ii) High-level molecular biology, including sequencing and sample/library preparation; (iii) working with murine models of immune dysregulation, including asthma, neuroinflammation, autoinflammation, infection, and cardiovascular disease; (iv) immune cells functional assays; (v) cloning and transfection; and (vi) screening primary patient cells for disease-causing mutations.

Must have a PhD degree (or foreign equivalent degree) in Biology, Immunology or a related field plus three (3) years of experience in a related position.

Experience must include three (3) years working with: (i) Human immunology research, including primary human immune cell isolation, culture, stimulation, gene editing, and functional analysis; (ii) Murine models of inflammation, allergy, and atherogenesis, including designing and conducting in vivo experiments and processing a wide range of tissues (aorta, lungs, GI, skin) for immunologic analysis, histological analysis, and specialized staining (i.e. aortic plaque staining);

(iii) Multiparameter flow cytometry, including experimental design, panel development, compensation, gating strategies, and data analysis; (iv) Molecular biology techniques, including nucleic acid extraction, qPCR, ddPCR, RNA-seq; (v) Gene-expression manipulation using lentiviral systems, including viral particles production and transduction for stable knockdown, knockout, or over-expression; (vi) Functional in vitro assays with mammalian cell lines, including evaluation of cell activation, viability, proliferation, cytokine production, etc;

and (vii) handling, shaving, and injecting (IV, IP, intradermal) mice and processing broad range of tissues.

Experience can be concurrent.

Apply at https://(Use the "Apply for this Job" box below). #.

The University of Pittsburgh is an equal opportunity employer.

Must have a PhD degree (or foreign equivalent degree) in Biology, Immunology or a related field plus three years of experience in a related position.