Assistant Research; PEJ--AI​/SAL-GL-; AM

Offer Description

IdiPAZ Seeksa research assistant in a research project co‑financed by the Community of Madrid and the European Social Fund Plus (ESF+), 2021‑2027, for the Pediatric Extracranial Solid Tumors group, led by Dr. Adriana Mañas, and part of the Translational Research in Childhood Cancer, Hematopoietic Transplantation, and Cell Therapy (GICITT) group, led by Dr. Pérez‑Martínez, within the Biomedical Research Foundation of La Paz University Hospital (IdiPAZ).

The candidate will participate directly in the projects led by Dr. Mañas, especially in the paediatric avatars project, focused on the creation and characterisation of patient‑derived models (in‑vivo and in‑vitro) and their use as avatars to develop new personalised treatments for paediatric solid tumours (neuroblastoma, hepatoblastoma, nephroblastoma and sarcoma).

IdiPAZ Health Research Institute is the result of a partnership between “La Paz” University Hospital, Universidad Autónoma de Madrid, the Healthcare Public Service of the Madrid Autonomous Region and the Foundation for Biomedical Research of La Paz University Hospital (FIBHULP), which is the managing body of the Institute. This strategic alliance has made it possible to tackle research projects through joint multidisciplinary teams, making the most of the human and material resources available to these entities for research and promoting research fundraising.

Our vision is to be a national and international reference for biomedical translational research of excellence, focus on people’s health.

E‑mail recursoshumanos

Research Field Biological sciences » Biology Education Level Master Degree or equivalent

The candidate will participate directly in the projects led by Dr. Mañas, especially in the paediatric avatars project, focused on the creation and characterisation of patient‑derived models (in‑vivo and in‑vitro) and their use as avatars to develop new personalised treatments for paediatric solid tumours (neuroblastoma, hepatoblastoma, nephroblastoma and sarcoma).

• Implantation of patient tumour samples in immunosuppressed mice (PDX models).
• Treatment of PDX models with chemotherapy and characterisation of tumour response by RNA analysis and histological techniques.
• Establishment of in‑vitro organoid models from PDX models and characterisation of the response to chemotherapy in vitro.
• Use in‑vitro (organoid) and in‑vivo (PDX) models to test and validate new therapies in comparison with standard chemotherapy.

The activities and functions to be performed by the candidate:

• Processing of patient tumour samples, both to preserve viability and for implantation in animals.
• Collaborate in the process of PDX implantation, monitoring and treatment of mice, and collection and processing of tumours for different analyses.
• Establish in‑vitro PDX‑derived tumour organoid cultures.
• Extraction and basic bioinformatics analysis of RNA from tumours and organoids.
• Processing of tumours and organoids for histological analysis.
• Treatment of tumour organoids with different therapies and analysis of viability and cytotoxicity.

• Previous experience in cell culture.
• Basic experience in biomolecular analysis techniques such as Western Blot, DNA/RNA extraction, PCR, etc.
• High level (C1) of spoken and written English.
• Advanced knowledge of Microsoft Office (Word, Excel, Power point).
• Previous experience in tumour organoid culture (positive assessment).
• Experience with paediatric cancer models.
• Possession of a certificate for working with animals (Functions A, B and
  
  C).
• Knowledge of specific software such as Graph Pad, Flow Jo or Image
  
  J.

Essential requirements for candidates to be eligible for the offer as set out in the call for applications:

• Be under 30 years of age on the date of contracting with the beneficiary entity.
• Be unemployed until the day before the date of contracting with the beneficiary entity.
• Have no other employment contract from the date of contracting.

Languages ENGLISH Level Excellent

• Contract for scientific and technical activities.
• Professional category of Higher Graduate or Graduate, Group II, Area 2, according to the…