R&D Assay Development Intern

Our group builds bioluminescent and fluorescent tools that let researchers see what happens inside living cells and in biochemical systems. As an intern, you will contribute to projects that may include:

• Bioluminescent reporter and complementation technologies:
  Nano Luc luciferase-based systems such as NanoBiT and HiBiT for measuring protein abundance, protein-protein interactions, and protein degradation.
• Immunoassay-based signaling detection: homogeneous, no-wash bioluminescent formats for measuring intracellular proteins and post-translational modifications such as phosphorylation.
• Fluorescence-based methods: fluorescent labeling (for example Halo Tag ligands), fluorescence imaging, and plate-reader fluorescence, used alongside luminescence for orthogonal validation.
• Enzyme activity assays: biochemical and cell-based detection of kinases and other signaling enzymes.
• Drug discovery applications: assay miniaturization, plate-based and high-throughput screening formats, and compound profiling.

• Perform cell culture, transfections, and cell-based assays in multi-well formats.
• Run biochemical assays, including reagent preparation, buffer optimization, and titrations.
• Operate luminometers, fluorescence plate readers, and imaging instruments.
• Collect, organize, and analyze data (dose-response curves, signal-to-background, Z’ factor, assay window.)
• Document experiments clearly in an electronic lab notebook.
• Follow protocols with direction, and learn to troubleshoot and ask the right questions when results are unexpected.
• Present results and contribute ideas at group meetings.

At Promega, we value diversity and strive to create an inclusive workplace. We are proud to be an Equal Opportunity Employer, making employment decisions without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, veteran status, disability, or any other protected class.