NAPE Research Technologist

Reporting to the Principle Investigator, the Research Technologist I is responsible for assisting with the study of elucidating the activity patterns of dopaminergic striatal neuronal circuits. In this study, a variety of genetically modified mice are used. Optimizing and benchmarking biological sensors in vivo is needed.

Assiston Research Projects- 75%

• Prepare shared stock solutions such as PBS and 4% paraformaldehyde.
• Operate histology experiment that includes mouse perfusion, brain sectioning, immunostaining, in situ hybridization, microscopy imaging, and analysis.
• Perform mouse behavioral studies and surgery under supervisor’s instruction.
• Compile the data and present to supervisor and schedule next experiment.

Mouse Colony Management- 20%

• Maintain compliance with UW animal care policies and monitor animal health, communicating with veterinary staff and taking appropriate action when concerns arise.
• Collaborate with the supervisor to develop and implement breeding strategies, including setting up breeding cages, monitoring mating success, and weaning pups.
• Perform mouse genotyping procedures, including ear punching, DNA extraction, PCR, electrophoresis, and image acquisition.
• Maintain accurate colony records in mLIMS, manage cage inventory and housing needs, coordinate cage transfers between facilities, and provide regular project updates to the supervisor.

Assist Lab Manager with Administrative Tasks - 5%

• Support daily laboratory operations and provide administrative assistance to the lab manager as needed.
• Maintain laboratory equipment and shared resources, including dishwashing and autoclaving laboratory glassware.
• Monitor, inventory, and replenish laboratory supplies to ensure uninterrupted research operations.
• Assist with onboarding and training new undergraduate students and postdoctoral scholars, and support administrative processes such as submitting packing slips to the finance department.

To be considered for this opportunity your application must demonstrate you meet both the minimum qualifications and additional qualifications listed below. Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Applicants who do not meet these qualifications WILL NOT be forwarded to the Hiring Department.

• Two years experience as a Laboratory Technician II, or equivalent; OR a Bachelors Degree in an appropriate field of science or technology.
• Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

• Experience with mouse care including handling, husbandry and familiar with animal use policies. Other procedures include administering euthanasia, performing perfusion, and sacrificing rodents.)
• Experience with mouse colony management including breeding and genotyping.
• Experience with histology experiment:
  Perfusion, sectioning with vibratome or cryostat, immunostaining or in situ hybridization, image acquisition, data analysis.
• Experience with handling chemicals.
• Having strong PC skills like Microsoft Office applications.
• Excellent in communication and teamwork.
• Excellent in time-management and organizing skills.

• Experience with a variety of scientific software’s. For image analysis, image J, Olympus Cell Sens, HALO and Zen. For data visualization, Corel Draw, Adobe illustrator and photoshop.
• Experience with writing code with Python or R.
• Experience with animal surgery and behavior experiment.

The Stuber lab is…