Research Lab Technician Associate

Michigan Medicine improves the health of patients, populations and communities through excellence in education, patient care, community service, research and technology development, and through leadership activities in Michigan, nationally and internationally.

Our mission is guided by our Strategic Principles and has three critical components; patient care, education and research that together enhance our contribution to society.

The Division of Pulmonary & Critical Care Medicine is seeking a Research Assistant or Laboratory Specialist for an existing laboratory located at the VA Medical Center Research Building. The Researcher will participate in ongoing research in a cell and molecular biology laboratory. This will involve a wide variety of techniques from the field of immunology/cell biology.

Duties include, but are not limited to:

* Under the direction of the principal investigator, initiate and perform experimental efforts entailing the following components:

Infect animals (mice) with microbes

Dissect the organs and tissues of infected animals

Isolate cell populations from mice.

Stain the cells with antibodies for flow cytometry experiments; acquire the data using our FACS system,

With provided gating strategy perform the analysis of leukocyte subsets (Flo Jo-program)

Isolate RNA for RT-PCR

Perform routine bioassays (ELISA, RT-PCR, CFU).

Perform various stimulated cell culture conditions over protracted time periods.

If experimental problems develop notify the PI/other personnel and assist in troubleshooting.

Record and enter the acquired data in notebooks and computer (excel and power point),

Ordering materials and supplies for the laboratory.

Assuring that all the equipment is in working order, if problems arise, assist in requesting repair services.

Prepare media, buffers, and solutions.

Generate accurate laboratory records, and supervise the purchasing and production of laboratory supplies

Prepare inventories of animals, chemicals and samples.

Other duties as assigned.

Bachelors degree in health or biological science discipline. Pre-PhD or pre-medical school applicants are encouraged to apply.

0-1 year experience

General understanding and preferably some experience with research in molecular biology, protein chemistry, immunology, and cell biology.

General understanding of biological and biochemical principles and practices

General understanding and preferably some experience biochemical laboratory techniques and instrumentation, such as preparing solutions, using analytical balances, pH meters, PCR, gel electrophoresis, spectrophotometers, coulter counters, scintillation counters, and centrifuges.

General understanding and preferably some experience of handling mice without reservation to handling the animals on daily basis.

General understanding and preferably some experience with cell culture techniques (animal).

Ability to work independently on assigned projects and analyses.

Ability to work effectively with supervisors and peers.

Previous experience in studying molecular biology, protein chemistry, immunology, and cell biology, Quantitative PCR, ELISA, staining cells with fluorescent monoclonal antibodies and flow cytometry experience, cell culture techniques, ability to work independently on pre-designed experiments and the initial data analysis.

Michigan Medicine is one of the largest health care complexes in the world and has been the site of many groundbreaking medical and technological advancements since the opening of the U-M Medical School in 1850. Michigan Medicine is comprised of over 30,000employees and our vision is to attract, inspire, and develop outstanding people in medicine,…