PhD student in Biofabrication​/Vascular Biology focus brain organoid vascularization

The Ente Ospedaliero Cantonale (EOC) and the Università della Svizzera italiana (USI) are seeking applications for the position of:

PhD student in Biofabrication/Vascular Biology with a focus on brain organoid vascularization The Regenerative Medicine Division is part of the Institute for Translational Research, Faculty of Biomedical Sciences, Università della Svizzera italiana (USI) and Ente Ospedaliero Cantonale (EOC), Switzerland. Strategic research areas of the Division are: in vitro disease modeling through biofabrication (e.g. age‑related diseases, cancer metastases, musculo‑skeletal diseases); design of novel technologies for drug screening;

personalized medicine applications using human tissue biopsies. To promote the advancement of these research areas, the Regenerative Medicine Division combines microfluidics and microphysiological systems, 3D (bio) printing and computational simulations.

We invite applications for a PhD position for conducting research in the project entitled “
Enhancing human brain organoid complexity through biofabrication of a perfusable vasculature
”.

The Division has recently granted funding from the Swiss National Science Foundation through a collaboration between Prof. Simone Bersini and Prof. Arianna Baggiolini (Institute of Oncology Research).

The 4 years project will deal with the biofabrication of miniaturized models of the human brain (i.e. organoids) and their integration with functional microvascular networks.

This cutting‑edge technology will allow to study key mechanisms of neural progenitor cell differentiation during brain development and to recapitulate increasingly complex architectures of the human brain.

The project involves the biofabrication of 3D microvascular networks mimicking the blood‑brain barrier, their integration with high‑throughput culture systems and the analysis of blood vessel‑brain organoid interaction through multiple next‑gen sequencing techniques and high‑content screening.

The Institute has access to cutting‑edge facilities (e.g. bulk and single‑cell RNAseq; spatial transcriptomics; mass spectrometry; confocal, multi‑photon and electron microscopy) which are shared with the Institute for Research in Biomedicine and the Institute of Oncology Research within a dynamic, multidisciplinary and collaborative environment.

The candidate will work under the scientific supervision of Prof. Simone Bersini ((Use the "Apply for this Job" box below).).

The successful candidate will be offered the possibility to work in a dynamic research team and in a multidisciplinary and international scientific environment. He or she will be enrolled in the Doctoral Program in Biomedical Sciences at USI.

The candidate will collaborate in the development of the institute research agenda. He or she will have shared responsibilities in the design, conduction and analysis of research projects in the field of biofabrication and molecular cell biology. In addition, the candidate is expected to present papers at scientific conferences and produce publications in high‑ranking journals.

Ideal candidates should satisfy the following requirements:

• A Master Degree in any Life Sciences or related disciplines, including Bioengineering. Priority given to Vascular Biology or Neuroscience background
• High personal interest in organ‑on‑a‑chip, biofabrication and (bio)‑printing
• Experience with (or strong commitment to quickly learn): 3D cell cultures (preferred experience with vascular cells and/or brain organoids), imaging (confocal microscopy, preferably with 3D cultures), standard biological techniques (qPCR, western blot, elisa, immunofluorescence), microfabrication of microphysiological systems, analysis of datasets including those from omic technologies
• Excellent skills in oral and written English
• Self‑motivation and exceptional commitment to experimental goals and deadlines
• Strong organizational skills and ability to work independently as well as in a team
• Critical data analysis and troubleshooting
• Effectively communicate experimental data, maintain records and write protocols

The successful candidate will work as a…