Scientist​/Senior Scientist, Library Prep

At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised >$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing.

What we are looking for in you

Glyphic is seeking a Scientist or Senior Scientist to lead research on the library preparation stage of our single-molecule protein sequencing platform. Reporting to the Director of Assay Development, you will own how DNA molecules produced by our protein sequencing assay (ProSE) are prepared for and read on the nanopore, the last steps of the assay through library prep to sequencing.

ProSE outputs are not standard sequencing templates and they impose constraints that off-the-shelf kits don’t address. As a successful scientist in this role, you will design creatively around those constraints and turn one-off gains in accuracy and read count into results the team can reproduce run after run.

The ideal candidate thinks about the whole system: how upstream chemistry, construct design, and what happens on a nanopore flow cell each shape the data. You have developed library prep methods for demanding templates and are comfortable working independently on open-ended problems. Direct nanopore experience is welcome but not required. We care more that you can learn a new platform quickly and reason from first principles about what you’re seeing.

This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location.

What you’ll do

Library preparation and method development :

• Own library prep method development for nanopore sequencing end to end: multiplexing, sample barcoding, second-strand synthesis, end repair, and adapter ligation. The goals are to maximize read count and efficiency and reduce run-to-run variability.
• Own the steps at the interface between our protein sequencing assay and library prep. Design and test new approaches to make accuracy gains reproducible.
• Develop on-bead preparation and clean-up methods for high-sensitivity sequencing from low-input material, along with characterization assays that track efficiency, yield, purity, and construct integrity at each stage.
• Feed library prep requirements back into the design of upstream constructs and oligos, working with the scientists who design them.
• Contribute to experimental design for ProSE studies and sequencing runs, and work with automation to move proven manual workflows onto automated platforms.

Mechanistic investigation and troubleshooting :

• Investigate and resolve yield-limiting artifacts from protein sequencing assay chemistry, and DNA library characteristics that reduce yield or bias results. This includes modified nucleotides and secondary structure during second-strand synthesis and end repair. Use computational tools (Snap Gene, mFold, NUPACK) and validate predictions at the bench.
• Use sequencing data as a measurement of the whole system. Work with bioinformatics to correlate library preparation variables with read counts, pore health, and classification accuracy, and design experiments that explain what you see.

Leadership and collaboration :

• Provide technical guidance and research experiment design to team members running routine library prep.
• Work closely with oligo synthesis, assay development, integration, automation, and bioinformatics teams to optimize the full sample-to-sequencer pipeline, and maintain rigorous documentation.

What you need

Required :

• PhD…