Research Technician Skilled In Mouse

Bethesda, MD

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Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision‑making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).

Axle is seeking a Research Technician Skilled In Mouse Work to join our vibrant team at the National Institutes of Health (NIH) supporting the National Human Genome Research Institute (NHGRI) located in Bethesda, MD.

Benefits We

Offer:

• Paid Time Off and Paid Holidays
• 401K match up to 5%
• Educational Benefits for Career Growth
• Employee Referral Bonus
• Flexible Spending Accounts:
  • Healthcare (FSA)
  • Parking Reimbursement Account (PRK)
  • Dependent Care Assistant Program (DCAP)
  • Transportation Reimbursement Account (TRN)

Position Overview

The Research Technician will provide research support to NHGRI scientists through laboratory methods, procedures, and techniques in cell biology, molecular biology, biochemistry, and glycobiology. The role includes working with mouse models of Free Sialic Acid Storage Disorder (FSASD) and GNE, supporting laboratory experiments and protocols, analyzing research data, and preparing results for presentation and publication.

Additional Qualifications

Education

• Bachelor's degree in Biology.

Certifications & Licenses

• Biosafety Level 1 (BSL
  1).

Software

Skills

• Primary cell culture
• Meet with laboratory members to present updates — Bi-Weekly

Statement of Work Details

• Work collaboratively with mouse models of FSASD and GNE in the NHGRI animal facility, including mouse breeding, observation, tissue collection, therapeutic testing, and documentation of breeding and tissue collection logistics.
• Support NHGRI scientists in planning, developing, and implementing laboratory experiments and protocols.
• Assist in coordinating communication with the Research Consortium on FSASD.
• Perform laboratory techniques in molecular biology, biochemistry, glycobiology, and cell biology, including sterile cell culture, polymerase chain reaction (PCR), quantitative reverse transcription PCR (qRT-PCR), deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) assays, cloning, protein assays, glycobiology tools, lectin technologies, and antibody techniques.
• Plan and execute in vitro and in vivo experiments, including cell culture, Western blotting, immunofluorescence, and immunohistochemistry.
• Perform primary cell culture, cell separation, and quantitative and qualitative analyses.
• Operate laboratory instruments, including confocal microscopes, spectrophotometers, plate readers, and other imaging equipment.
• Process specimens for embedding according to established protocols.
• Maintain sample inventories and storage according to outlined guidelines.
• Analyze, document, and interpret experimental data; discuss results with laboratory team members.
• Prepare experimental progress updates, reports, summaries, and written, tabular, and graphical presentations of findings.
• Prepare data and conclusions for publication in professional journals, including publication-quality graphs.
• Maintain laboratory supplies and consumables, track supply usage, and initiate purchase orders.
• Clean and sterilize instruments, glassware, and equipment; support glassware washing, media preparation, and autoclaving.
• Perform laboratory equipment maintenance and coordinate liquid waste container pickup.
• Provide technical…