Automation Cell Culture Biologist

Job Details:
Level: Experienced,

Job Location:

National Institutes of Health (NIH) - Main Campus/Bethesda - Bethesda, MD 20892, Position Type:
Full Time, Education Level: Masters Degree, Salary Range: $85000.00 - $115000.00

Salary/year, Travel Percentage:
None, Job Shift: Day, Job Category:
Public Health, Position Objective:
This Automated Cell Culture Biologist supports research at the Center for Alzheimer's and Related Dementias at the National Institute on Aging (NIA) within the National Institutes of Health. This position characterizes genetic pathways for disease by automating large scale cell culture experiments.

• Plan and administer biological research programs
• Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function
• Develop and maintain liaisons and effective working relations with groups and individuals, agencies, and the public to encourage cooperative management strategies or to develop information and interpret findings
• Work with staff to characterize genetic pathways for Alzheimer’s disease and related dementia (ADRD) using human cell-based models.
• Work with staff on performing large-scale iPSC cell culture experiments on liquid handling platforms.
• Program, operate, and implement novel methods on liquid handling platforms (Hamilton, Tecan).
• Work with staff on performing various experiments and procedures related to differentiation and phenotyping of induced pluripotent stem cell lines.
• Evaluate the stability of iPSC and iPSC-derived cells across multiple engineered and non-engineered lines.
• Work with staff to apply published protocols for cell differentiation to IPSC lines, microscopy, and molecular analysis.
• Work independently in designing, developing, and performing new methods
• Work with staff to provide insight into the effects of ADRD-associated mutations on molecular readouts in cell culture.
• Support other research projects.
• Collect, Analyze, interpret, and organize data and present results in team and departmental meetings.
• Keep detailed, organized, and accurate digital and physical experimental records.
• Update and maintain laboratory notebooks and databases.
• Attend meetings and present results and findings for staff discussion.
• Collect and analyze biological data about relationships among and between organisms and their environment
• Monitor and observe experiments, recording production and test data for evaluation by research personnel
• Analyze experimental data and interpret results to write reports and summaries of findings
• Coordinate the work of biological technicians and technologists and other scientists
• Collaborate with other team members to carry out large-scale cell culture protocols using high throughput robotic systems
• Perform standard immunological techniques, including western blotting and immunostaining.
• Operate, calibrate, or maintain equipment used in quantitative or qualitative analysis, such as spectrophotometers, calorimeters, flame photometers, or computer-controlled analyzers
• Maintain laboratory equipment
• Operate laboratory equipment to analyze samples 5
• Analyze laboratory findings
• Train staff on the proper usage of laboratory instruments and equipment.
• Calibrate, maintain, and troubleshoot instruments and equipment in the laboratory.
• Maintain and perform staff training on scientific instrumentation, cell culture micros copies, microanalytical balances, centrifuges, automated platforms.
• Operate, troubleshoot, and train others to use laboratory equipment and software.
• Provide assistance to laboratory staff in designing experiments, using instrumentation, data acquisition and analysis, and preparing figures for publication
• Maintain scientific equipment and resources including reagents, stocks of…