UNIV - Research Specialist III - Department of Pharmacology & Immunology

We seek a fulltime Research Specialist III for the experimental bone marrow transplantation laboratories and tumor immunology laboratory of Dr. Paczesny in the Hollings-NCI designated Cancer Center. The primary responsibilities will include pre-clinical models of Graft-versus-Host disease and acute myeloid leukemia. Specifically, this will involve daily observation and scoring of animals, procurement and breeding of experimental animals and coordination of their housing and care.

General laboratory responsibilities will include supply procurement, document maintenance and work closely with IACUC and various other agencies to ensure safety and regulatory compliance for the laboratories. In addition, the candidate will actively participate in setting up transplants (including marrow isolation, spleen cell purification, irradiation and /or chemotherapy and small animal handling/injection) as well as in vitro cellular immunology experiments including CTL assays, MLR cultures, ELISAs, and FACS analysis including spectral flow.

Entity Medical University of South Carolina (MUSC - Univ) Worker Type Employee Worker Sub-Type Research Grant Cost Center CC001032 COM PHARM Administration CC Pay Rate Type Salary Pay Grade University-GEN
09 Pay Range 53,142.

- 90,372.000 Scheduled Weekly Hours 40 Work Shift

• Perform immunologic experiments on murine models (e.g., harvest organ, digest, flow cytometry, confocal, etc) (20%).
• Immunology experimentation employing various techniques, including isolation and purification of T cells, and regulatory T cells. Sort T cells and other hematopoietic or immunes cells. Perform isolation of cells, cell culture and in vitro functional assays (20%).
• Harvest murine organs, including spleen, liver, bone marrow, and lungs. Perform tissue digestion and preparation of single-cell suspensions. Perform isolation and purification of T cells, and regulatory T cells. Sort T cells and other hematopoietic or immunes cells. Maintain cell lines; viability, cytotoxicity assay; transfection and infection, etc. Perform routine handling of mice including monitoring, treatments, and experimental procedures) (20%).
• Assist with breeding, colony management, and genotyping of transgenic. Maintain accurate animal records and ensure compliance with institutional animal care and biosafety protocols.
• Assist with intravenous and intraperitoneal injections of novel therapeutics (cellular of antibody-based). Records and analyzes results of research experiments (15%).
• Collect research experimental data and analyze results and generate figures. Assist with daily lab operations including routine maintenance of and experimentation in mice (15%).
• The selected candidate will assist with daily lab operations including stocking consumable items and reagents, making buffers, and aliquoting reagents. Fill liquid nitrogen to keep frozen cells alive. Autoclave the biologic trash and prepare and maintain lab work (e.g., fill and autoclave tips, clean hood, and incubator). Facilitate general maintenance of the research space by working with MUSC to ensure that the laboratory area is maintained and that repairs, waste disposal, and cleaning needs are addressed in a timely manner.
• Additional duties assigned by the Principal Investigator (10%). This will include helping refine or prepare lab protocols, maintain IBC and IACUC protocols up to date. Train new lab members for basic experiments.

Candidates with a master degree and minimal 7 years’ experience in the field of immunology, microbiology, molecular biology, cell biology or biochemistry are encouraged to apply. Ability to work independently and good management skills are required. This position requires…