Flow Cytometry Tech

The DFCI Cytometry Cores provide expertise and access to cutting‑edge conventional and spectral Flow Cytometry and Mass Cytometry in a shared‑use setting. The Cytometry Technologist I will receive on‑the‑job training in performing flow cytometric analysis, sorting and immunophenotyping by mass cytometry (CyTOF). We are seeking a dynamic technician to assume responsibilities including the operation, calibration and maintenance of Cell Sorters and Analyzers along with an XT Mass Cytometer, data acquisition, analysis, and documentation of results.

Participate in the development and validation of new procedures and tests in the laboratory. Becoming part of this Core’s team enables you to provide the highest quality scientific service to the Dana Farber Research Community.

Located in Boston and the surrounding communities, Dana‑Farber Cancer Institute is a leader in life‑changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS, and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high‑risk and underserved populations.

We conduct groundbreaking research that advances treatment, we educate tomorrow’s physician/researchers, and we work with amazing partners, including other Harvard Medical School‑affiliated hospitals.

• Operation and maintenance of fluorescence‑activated cell analyzer and cell sorters including data acquisition for patient and research samples, calibration, preventative maintenance and troubleshooting.
• Operation and maintenance of cytometer‑related computer hardware and software.
• Preparation and immunophenotyping of human cellular products for use in research protocols according to lab procedures.
• Preparation and maintenance of cytometry‑related reagents.
• Data analysis, documentation and maintenance of test results according to lab procedures.
• Perform tests using SOP involving multiple methodologies. These may include automated, semi‑automated or manual technologies.
• Prepares cellular products for biological standards.
• Performs cell culture & aseptic technique for cell sorting.
• Review and retrieve samples for special studies or additional testing.
• Accurately produce and document the processing data.
• Achieve expected results within established turnaround times for cell sorting products that support research care goals.
• Detect, troubleshoot, and correct instrument, reagent, and system failures.
• Perform preventive and corrective maintenance of equipment and instrumentation. Identify the need for repair or referral as required. Calibrate equipment as scheduled.
• Evaluates/troubleshoots problems related to product analysis and preparation. Communicates outcomes to appropriate supervisor.
• Evaluate test results & analyze an assay for acceptability.
• Other duties and procedures as required or assigned.

• Performs quality control duties.
• Perform and document QC/QA activities as defined in policies & SOPs.
• Participate in validation of new instruments & methods.
• Examine existing processes and problems and continuously look for way to improve them. Appropriate follow up & documentation.
• Participate in development of new procedures and continuous improvement activities.
• Perform & report proficiency & competency testing as required.
• Maintains records of procedures and resultant data, both manually and on computer.
• Work within the Flow Cytometry Core guidelines and adheres to all regulatory standards within the Flow Cytometry Core Facility.
• Maintains and prepares laboratory work records.
• Ability to enter & retrieve data from computer data bases & spreadsheets.
• Other general administrative duties as required.

• Foster adherence to safety compliance and exemplifies proper behavior in this regard.
• Attend safety training as required. Safety compliance: follow institution's safety procedures, wear PPE. Know, understand & adhere to all safety standards, i.e., emergency…