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Associate Scientist - Nuclease Engineering

Job in Boston, Suffolk County, Massachusetts, 02298, USA
Listing for: Eli Lilly and Company
Full Time position
Listed on 2025-12-02
Job specializations:
  • Science
    Research Scientist, Data Scientist
Job Description & How to Apply Below
Associate Scientist - Nuclease Engineering

Join to apply for the Associate Scientist - Nuclease Engineering role at Eli Lilly and Company

Associate Scientist - Nuclease Engineering

Join to apply for the Associate Scientist - Nuclease Engineering role at Eli Lilly and Company

At Lilly, we unite caring with discovery to make life better for people around the world. We are a global healthcare leader headquartered in Indianapolis, Indiana. Our employees around the world work to discover and bring life-changing medicines to those who need them, improve the understanding and management of disease, and give back to our communities through philanthropy and volunteerism. We give our best effort to our work, and we put people first.

We’re looking for people who are determined to make life better for people around the world.

Eli Lilly and Company is seeking a Therapeutic Editing Associate Scientist to join our team in Boston, MA.

Come join the rapidly expanding Gene Therapy team at Eli Lilly! We are responsible for inventing, characterizing, and advancing novel genome editing reagents for a myriad of clinical applications. Our team works cross-functionally within our organization across multiple disease areas and platforms. Our goal is to deliver on the promise of gene editing to patients around the world.



Job Responsibilities

  • Collaborate with team members to engineer next-generation gene editors with enhanced activity and improved safety profiles.
  • Support the design, engineering, and optimization of novel nucleases using techniques such as site-directed mutagenesis, structure-guided design, and directed evolution.
  • Develop and implement new assays to measure editing efficiency of gene editors (e.g., CRISPR) in vitro, in cell-free systems, and in bacterial models.
  • Work cross-functionally to deliver data to the project team within the expected timelines.
  • Maintain detailed documentation of scientific work in an electronic notebook.
Basic Qualifications

  • Bachelors and/or Master’s degree in biology or a closely related scientific field with 3+ years of relevant laboratory work experience.


Additional Skills/Preferences

  • Extensive experience with bacterial screening systems and aseptic cell culture techniques, including hands-on execution of CRISPR-based gene editing experiments.
  • Prior experience in Nuclease engineering within an industrial or biotech setting, contributing to the development of gene editing therapies.
  • Expertise in protein engineering, including structure-guided and rational design approaches, as well as experience with bacterial or yeast-based directed evolution systems.
  • Proficient in molecular biology workflows such as bacterial transformation, transfection, PCR, ddPCR, restriction digestion, Gibson assembly and other molecular cloning workflow.
  • Experience with large cargo editor and Cas-fusion proteins, as well as a desire to learn to apply new and emerging technologies
  • Experience planning and executing experiments independently
  • Familiarity with primer design, sequencing preparation, and analysis
  • Experience using automation in workflows is a plus (i.e., liquid handling)
  • Excellent problem-solving skills and ability to troubleshoot complex issues
  • Strong organization, communication, and collaboration skills
Additional Information

  • This role may require up to 10% travel for conferences or training.
Lilly is dedicated to helping individuals with disabilities to actively engage in the workforce, ensuring equal opportunities when vying for positions. If you require accommodation to submit a resume for a position at Lilly, please complete the accommodation request form () for further assistance. Please note this is for individuals to request an accommodation as part of the application process and any other correspondence will not receive a response.

Lilly is proud to be an EEO Employer and does not discriminate on the basis of age, race, color, religion, gender identity, sex, gender expression, sexual orientation, genetic information, ancestry, national origin, protected veteran status, disability, or any other legally protected status.

Our employee resource groups (ERGs) offer strong support networks for their members and…
Position Requirements
10+ Years work experience
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