Scientist III iPSC-Derived Gut Models

The Scientist III – (iPSC-Derived Gut Models) will play a key role in developing, optimizing, and applying advanced human in vitro gut model systems to support preclinical research and therapeutic discovery. This position focuses on iPSC-derived intestinal models and combination therapy studies to better understand disease biology and treatment responses. The Scientist III will work in a highly collaborative, cross‑functional environment, integrating cellular biology, immunology, and pharmacology to generate decision‑enabling data that supports translational research efforts.

• Develop, optimize, and execute iPSC‑derived gut model systems, including 3D intestinal organoids, epithelial monolayers, and microphysiological platforms
• Perform iPSC maintenance and differentiation into intestinal epithelial and endodermal lineages
• Establish and run co‑culture systems with immune cells (e.g., macrophages, dendritic cells, T cells) to model inflammatory disease contexts
• Design and execute mono‑ and combination‑therapy studies, including dose–response and time‑course experiments using biologics and small molecules
• Apply quantitative functional readouts such as barrier integrity (TEER, permeability), wound healing, mucus layer assays, and cytokine profiling
• Conduct inflammatory and cell‑state analyses using ELISA/MSD, qPCR/RT‑qPCR, flow cytometry, and imaging‑based techniques
• Collaborate with data science partners on transcriptomic profiling (bulk and/or single‑cell) and quantitative data analysis
• Implement genetic and pharmacologic perturbations to interrogate mechanisms and predict combination responsiveness
• Assess assay reproducibility, dynamic range, and throughput; adapt workflows to plate‑based and automated formats
• Present experimental plans, results, and project updates to internal stakeholders and senior leadership while maintaining rigorous documentation and SOPs
• Ensure compliance with laboratory safety standards and proper maintenance of cell lines, reagents, and equipment

• Master’s degree with 5+ years of relevant experience or
• PhD with 1–2+ years of relevant experience
• Field of study:
  Molecular Biology, Cellular Biology, Immunology, Pharmacology, or related discipline

Hands‑on experience with human cell culture and at least one of the following:

• iPSC maintenance and differentiation into epithelial/endodermal lineages
• Intestinal organoids (enteroids) or epithelial monolayers
• Proven ability to design, execute, and troubleshoot functional assays, including dose–response studies
• Strong data analysis, documentation, and scientific presentation skills
• Demonstrated success working in collaborative, cross‑functional research environments
• Excellent verbal and written communication skills

• Direct experience with iPSC‑derived intestinal models (3D organoids, 2D monolayers, Transwells, gut‑on‑chip systems)
• Expertise in barrier function assays, imaging‑based analyses, and cytokine profiling
• Experience with automation, liquid handling, and high‑throughput assay development
• Background in inflammatory disease biology
• Prior industry experience in preclinical pharmacology or combination therapy testing