Scientist, Proteomics Cambridge, MA

We are seeking a highly motivated and talented scientist to join Company’s Chemical Biology and Proteomics group within Discovery Sciences. You will apply proteomics to advance drug discovery
. You will work as an integral member of a cross-functional team to interrogate mechanisms of action for therapeutic agents, and to propose and validate biological hypotheses for agents within the c
ardiovascular, renal and metabolism (CVRM) area.

• Design and execute discovery proteomic assays using mass spectrometry to support mechanistic studies, Client Client targets, and elucidate biological regulators of disease biology.
• Perform proximity biotinylation studies in cells, using established automated affinity purification workflows, and data independent acquisition (DIA) mass spectrometry methods.
• Perform target validation by developing proximity ligation assays (PLA) and cell-based assays to assess protein-protein interactions using chemical tools and confocal microscopy.
• Generate protein conjugates and characterize protein labeling by UV-Vis spectroscopy.
• Analyze and interpret experimental data using established bioinformatic pipelines.
• Maintain detailed and accurate electronic laboratory notebooks.
• Represent CBP team, and communicate with project discipline leads, and contribute to generation of mechanistic hypothesis through interpretation of proteomics data.
• Ph.D. in Proteomics, Chemical Biology, Biological Sciences, Biochemistry, or a related discipline, with at l
  east 2 years of hands-on proteomics experience
  , including experience gained during doctoral or postdoctoral research. Or Masters degree with 3-5 years of hands-on proteomics experience.
• Knowledge and hands-on experience with proteomic sample preparation.
• Experience in mammalian cell culture
  .
• Experience employing cell-based and proteomic assays to understand small molecule mechanisms of action.
• Demonstrated ability to work effectively both independently and collaboratively, contribute to scientific discussions, solve problems, and deliver high-quality results within agreed timelines.

• Experience developing or applying proximity ligation assays or related proximity-based protein-protein interaction validation assays.
• Experience using LC-MS/MS platforms and semi-automated instrumentation.
• Experience using proteomics search and analysis tools, such as Spectronaut, Proteome Discoverer, Max Quant, Skyline and Xcalibur.