Scientist Bioscience - Proteomics

Location: Cambridge, MA

Onsite Flexibility: Onsite

• Position Type: Contract
• Contract Duration: 12 months
• Pay Rate: $40.00–$48.00 / Hour (USD)
• Work Authorization: Applicants must be authorized to work for ANY employer in the U.S. We are unable to sponsor or take over sponsorship of an employment Visa at this time.

We are seeking a highly motivated and talented scientist to join a global biopharmaceutical organization's Chemical Biology and Proteomics group within Discovery Sciences. As a Research Scientist on a 12-month fixed-term contract, you will apply proteomics to advance drug discovery. You will work as an integral member of a cross-functional team to interrogate mechanisms of action for therapeutic agents, and to propose and validate biological hypotheses for agents within the cardiovascular, renal and metabolism (CVRM) area.

If you're inspired by the possibilities of science to make a difference and ready to discover what you can do join us.

• Design and execute discovery proteomic assays using mass spectrometry to support mechanistic studies, identify targets, and elucidate biological regulators of disease biology.
• Perform proximity biotinylation studies in cells, using established automated affinity purification workflows and data-independent acquisition (DIA) mass spectrometry methods.
• Perform target validation by developing proximity ligation assays (PLA) and cell-based assays to assess protein-protein interactions using chemical tools and confocal microscopy.
• Generate protein conjugates and characterize protein labeling by UV-Vis spectroscopy.
• Analyze and interpret experimental data using established bioinformatic pipelines.
• Maintain detailed and accurate electronic laboratory notebooks.
• Represent the CBP team, communicate with project discipline leads, and contribute to the generation of mechanistic hypotheses through interpretation of proteomics data.

• Hands-on proteomics experience (from graduate, postdoctoral, or industry work)
• Mammalian cell culture
• Data analysis
• Knowledge and hands-on experience with proteomic sample preparation
• Experience employing cell-based and proteomic assays to understand small molecule mechanisms of action
• Demonstrated ability to work effectively both independently and collaboratively, contribute to scientific discussions, solve problems, and deliver high-quality results within agreed timelines
• Similar workflows including mammalian cell culture, protein conjugation, purification, and UV-Vis spectroscopy characterization
• Affinity enrichment proteomics and mass spectrometry: carrying out advanced mass spectrometry-based proteomics with affinity enrichments
• Expected to operate with significantly higher independence than the Associate Scientist

• Experience developing or applying proximity ligation assays or related proximity-based protein-protein interaction validation assays
• Experience using LC-MS/MS platforms and semi-automated instrumentation
• Experience using proteomics search and analysis tools, such as Spectronaut, Proteome Discoverer, Max Quant, Skyline, and Xcalibur

• Ph.D. in Proteomics, Chemical Biology, Biological Sciences, Biochemistry, or a related discipline, with at least two years of hands‑on proteomics experience, including experience gained during doctoral or postdoctoral research
• OR a Master's degree with 3–5 years of hands‑on proteomics experience (minimum 1 year of industry proteomics experience)

• Hands-on proteomics experience is mandatory (from graduate, postdoctoral, or industry work)
• Experience in mammalian cell culture
• Ph.D. candidates: at least two years of hands‑on proteomics experience, including…