Postdoctoral Fellow or Associate Staff Scientist; Peters) Liver Fibrosis

## Postdoctoral Fellow or Associate Staff Scientist (Peters) Liver Fibrosis Apply:
Burnet Campus:
Full time:
Posted Today:
JR225568
** Primary Location
** Location T
** Department
* * Gastroenterology
* * Shift
* * Day (United States of America)
** Schedule
* * Full time
** Weekly Hours
** 40
* * FTE
* * 1
* * Employee Status
** Regular  Alexander Miethke’s Lab has an immediate opening for a Postdoctoral  Research Fellow or Associate Staff Scientist in the Division of Gastroenterology to study mechanisms driving immune mediated liver injury, bile duct damage and liver fibrosis in pediatric autoimmune liver disease. Dr. Miethke’s research focuses on discovering immune pathways controlling hepatobiliary injury in primary sclerosing cholangitis (PSC) and autoimmune hepatitis (AIH) in order to ultimately develop novel therapies (https://www.cincinnatichildrens.org/bio/m/alexander-miethke).

The laboratory uses established mouse models for sclerosing cholangitis, and several knockout and transgenic mice to discern disease mechanisms in pre-clinical models and works closely with the Center for Autoimmune Liver Disease. (https://www.cincinnatichildrens.org/service/a/autoimmune-liver-disease).  Dr. Miethke collaborates with Dr. Wang, computational biologist in the division (https://www.cincinnatichildrens.org/research/divisions/g/gastroenterology/labs/wang) and with Dr. Peters who studies mechanisms of liver allograft rejection and tolerance (https://www.cincinnatichildrens.org/bio/p/anna-peters). The specific goals for the successful applicant are
1) to refine iPSC-derived cellular co-culture and multicellular organoid systems to recapitulate liver tissue single-cell RNAseq predictions on cellular cross talks driving liver cell injury and biliary fibrosis in PSC and AIH,
2) to develop animals models of large duct PSC, and
3) to prepare high quality single cell and single nuclear preparations from cryopreserved liver (allograft) tissue samples for single cell RNA/ATAC seq.  The ideal candidate will have a PhD or equivalent in biomedical research. Experience in basic molecular techniques including gene expression quantification (RT-PCR), protein analysis (ELISA, Western), immunohistochemistry, high parameter flow cytometry, cell-culture techniques with iPSC-derived and transformed cell lines, and established procedures for mouse model systems are required.

Candidates with a strong background in bioinformatics, and mouse/human immunology are encouraged to apply.

Experience with analysis of RNAseq data and multi-parameter flow cytometry is preferred.  
** JOB RESPONSIBILITIES**
* ** Conduct Research/Lab Administration
- ** Plans, directs and executes scientific research and laboratory experiments. Investigates the feasibility of applying a variety of scientific principles and concepts to solve problems. Works on complex problems in which analysis of situations or data requires an in-depth evaluation of various factors, applying broad knowledge of principles, theories and standard scientific/research protocols. Uses professional concepts in accordance with objectives to solve complex problems in creative and effective ways.

Exercises judgment within broadly defined practices and policies in selecting methods, techniques and evaluation criteria for obtaining results. Responsible for lab administration (IRB/IACCUC/biosafety). May oversee divisional or institutional core facilities, managing and/or providing training for instrumentation. Research and improve current methods and evaluate innovative techniques. Design and perform experiments and record data according to research protocol. Independently reproduce, evaluate, research, and improve techniques currently used.

Adapt new methods to existing procedures and…