Research Scientist , Pisu Lab

Summary

As a Research Scientist I will be responsible for advancing NIH- and startup-funded studies on Mycobacterium tuberculosis. The scientist will independently design and execute molecular, cellular, and microbiological experiments including cloning, CRISPR/Cas9 editing, next‑generation sequencing (NGS) library preparation, flow cytometry and cell sorting, microscopy (confocal and fluorescence), and protein assays (Western blot, ELISA, Olink). The position requires handling of bacterial cultures (E.

coli, M. bovis BCG, M. smegmatis, and virulent M. tuberculosis), macrophage infection models, and lentiviral systems for CRISPR delivery. The role will begin with BSL-2 work and transition to BSL-3 pathogen culture and murine infections once clearance is granted. The Research Scientist I will also maintain compliance with IACUC and biosafety protocols, contribute to publications and grant proposals, and assist in training additional staff and students.

• Design and execute molecular, cellular, and microbiological experiments.
• Perform cloning, CRISPR/Cas9 editing, NGS library prep, flow cytometry and cell sorting, microscopy, and protein assays.
• Handle bacterial cultures (E. coli, M. bovis BCG, M. smegmatis, virulent M. tuberculosis), macrophage infection models, and lentiviral CRISPR delivery.
• Begin work in BSL-2 and transition to BSL-3 pathogen culture and murine infections upon clearance.
• Maintain compliance with IACUC and biosafety protocols.
• Contribute to publications and grant proposals.
• Assist in training additional staff and students.

• Technical skills and competencies to conduct quantifiable, meaningful research.
• Excellent oral, written, and interpersonal communication skills.
• Ability to interact with individuals of diverse backgrounds at all levels, internal and external to the organization.
• Ability to work collaboratively as a positive, contributing member of a team.
• Ability to keyboard and utilize various standardized word processing, spreadsheet preparation, and presentation software.
• Ability to manage multiple tasks and projects simultaneously.
• Ability to prioritize work, performing assignments with minimal supervision.

• Terminal Degree (Ph.D./M.D./D.V.M., etc.)

• Work experience in an academic environment.