Research Associate, Research Scientist, Clinical Research

Research Associate page is loaded## Research Associate locations:
Dallas, TXtime type:
Full time posted on:
Posted 2 Days Agojob requisition :
JR5608

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** Job Posting

Title:

** Research Associate
*
* Location:

** Dallas - Hospital
** Additional Posting Details:
** Monday - Friday 8:00a-4:30p
*
* Job Description:

**** Duties/Responsibilities
**** Cell Processing and Culture
*** Perform processing of blood and bone marrow samples, including filtration, Ficoll gradient separation, and magnetic-activated cell sorting (MACS).
* Isolate and culture bone marrow stromal cells (BMSCs); perform osteogenic induction and staining procedures such as Alizarin Red and alkaline phosphatase (ALP) staining.
* Maintain macrophage cultures, including routine medium changes, stimulation, detachment/harvesting, and cryopreservation.
** Molecular Biology and Genetic Engineering
*** Apply molecular biology techniques such as single-cell cloning and cell line development.
* Perform genome-editing experiments using CRISPR/Cas9 systems, RNA interference (RNAi), or other gene-modulation tools.
* Validate edited or cloned cells through PCR genotyping, sequencing, and phenotype-based assays.
* Contribute to optimization of cloning, transfection, and gene-editing workflows.
** Imaging and Instrumentation
*** Conduct micro-CT scanning, image reconstruction, and quantitative data analysis.
* Utilize microscopes for live/dead viability staining, routine culture monitoring, and morphological assessments.
** Molecular and Biochemical Assays
*** Perform RNA extraction, cDNA synthesis, and quantitative PCR (qPCR) for gene-expression analysis.
* Conduct biochemical assays including ELISA, BCA protein quantification, Western blot analysis, and MTT viability assays.
* Prepare samples, reagents, and staining panels for flow cytometry (FACS) analysis.
** Software and Data Analysis
*** Analyze imaging and experimental data using CTAn, CTVolX, Flow Jo, Image

J/Fiji, Excel-based qPCR analysis tools, and Graph Pad Prism.
* Maintain accurate electronic records and generate figures, data summaries, and reports.
** Laboratory Operations
*** Prepare, track, and manage chemical, reagent, and supply orders; complete order forms and submit purchasing requests.
* Assist with maintaining laboratory organization, equipment maintenance, and routine quality-control documentation.
** Required Skills/Abilities
*** Bachelor’s degree with
** 10 years
** of laboratory experience, or Master’s degree with
** 5 years
** of laboratory experience required.
* Strong
** critical thinking
** and
** time-management
** skills.
* Ability to
** multi-task
** and work with
** minimal supervision
** in a research environment.
* ** Experience with cell cloning and gene editing is preferred.
**** Working Environment
**
* Working Conditions:

Inside
* Working Position:
Sitting, Standing, and Walking
* Physical Demands:
Light

* Physical Requirements:

Repetitive Movements
* Lifting or carrying up to 10 lbs
* Sensory/Communicative activities essential to the performance of this position:
Hearing, Seeing, and Speaking
* This position will be exposed to Inside Environments, Bloodborne Pathogens and Respiratory Hazards
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