Research Technician II

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School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe.

Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe.

The Department of Molecular Genetics and Microbiology at Duke University School of Medicine is seeking a research technician with near-immediate availability. The position will be for one year to start with extensions contingent on availability of funding, performance, and needs of the lab.

This position will involve performing a variety of technical and research duties to support the laboratory of Dr. John Rawls (https://sites.duke.edu/rawlslab/ ). Dr. Rawls’ lab uses zebrafish, mice, and bacterial model systems to investigate mechanisms of host-microbiome interactions in the intestine, and their implications for digestive physiology, nutrition, metabolism, inflammation, and inter-organ communication.

The successful candidate will have the demonstrated ability to perform, or be willing to learn, the following tasks in a research laboratory:

• Weekly tasks managing mouse colony maintained by the Rawls Lab. This will include mouse manipulation, blood/fecal/tissue collection, strain maintenance and database management, DNA extraction, primer design, PCR, and gel electrophoresis. The candidate will be expected to document animal colony status in an organized, complete, and accurate fashion.
• Daily tasks conducting research on zebrafish endocrinology. This will include zebrafish manipulation, blood and tissue collection, histology, immunofluorescence, immunohistochemistry, microscopy, image analysis, RNA and protein extraction, ELISA, and Western blot. The candidate will be expected to document procedures and collect data in an organized, complete, and accurate fashion.
• Ancillary laboratory maintenance tasks including the preparation of culture media, chemicals, reagents and solutions, using basic computer skills (Word, Excel, Powerpoint), attending weekly lab meetings, and clearly presenting and visualizing data as it pertains to experimental outcomes and colony maintenance.
• Ancillary support tasks include assisting faculty, postdocs, students, and staff in the development and execution of new research procedures and techniques. This would include providing support for zebrafish and mouse experiments.

Education: Work generally requires a bachelor's degree in…