Scientist, Research Scientist

Job Description

Pay Range: $55.02hr - $60.02hr

• Design and construct expression vectors using advanced molecular cloning techniques including Gibson assembly, Golden Gate cloning, restriction enzyme-based cloning, and site‑directed mutagenesis.
• Develop, optimize, and implement automated workflows for high-throughput vector construction and DNA assembly on liquid handling platforms.
• Generate, validate, and bank vector constructs for expression of candidate biotherapeutic molecules in mammalian cell systems.
• Perform molecular characterization of cell lines and constructs using techniques such as ddPCR, Sanger sequencing, and restriction mapping.
• Support NGS library preparation for genomic and transcriptomic analysis of CHO cell lines.
• Optimize and troubleshoot molecular biology protocols to improve efficiency and reproducibility.
• Prepare technical reports and presentations to communicate progress and data.

• PhD (0‑3 years) in Molecular Biology, Cell Biology, Biochemistry, Bioengineering, or related discipline.
• Extensive hands‑on experience with molecular cloning techniques including Gibson assembly, Golden Gate cloning, restriction cloning, and PCR-based methods.
• Demonstrated expertise in DNA construct design, vector engineering, and plasmid preparation.
• Experience with genetic characterization methods such as ddPCR, qPCR, gel electrophoresis, and DNA sequencing analysis.
• Proficiency in designing and optimizing molecular biology workflows for automation on liquid handling platforms (Hamilton, Tecan, or similar).
• Strong technical background in molecular biology with excellent attention to detail and troubleshooting skills.
• Self‑motivated with excellent organization, time‑management, and communication skills.
• Maintain detailed electronic laboratory notebooks and documentation of all experimental procedures.
• Demonstrated ability to work as member of a team and adhere to timelines.

• Mammalian cell culture experience in a pharmaceutical or biotechnology setting, particularly with CHO cells.
• Experience with high‑throughput screening and clone selection strategies.
• Familiarity with bioinformatic tools for DNA sequence analysis, primer design, and in silico cloning (Snapgene, Geneious, Benchling, Primer3 or similar).
• Knowledge of CHO cell line development processes and recombinant protein expression.
• Experience using Oxford Nanopore Technologies in NGS workflows.
• Basic programming skills in R or Python for data analysis and visualization.
• Experience with Bash/command line scripting for bioinformatics workflows and data processing.
• Experience with automated colony picking and clone tracking systems.
• Flexibility to adjust to a fast‑paced, goal‑oriented environment and willingness to expand and broaden skillset.