Senior Research Asssociate

Job Title:
Senior Research Associate

Location:
Boston, MA

Pay Range: $44 - $46/hr on W2
Work Arrangement:
Fully Onsite
Employment Type:
Contract
Duration: 1 year

We are seeking a skilled Senior Research Associate to join a molecular biology and analytical development team. This individual will lead hands-on single-cell RNA sequencing (scRNA-seq) sample processing and library preparation to support clinical research programs.

The ideal candidate will have practical experience with single-cell genomics workflows, particularly 10x Genomics Chromium, and the ability to independently execute experiments, troubleshoot technical challenges, optimize protocols, and collaborate with cross-functional scientific teams.

• Plan, execute, document, and troubleshoot single-cell RNA sequencing experiments using established genomics platforms.
• Perform cell and tissue dissociation, cell counting, viability assessment, enrichment, cryopreservation, and preparation of single-cell suspensions from primary cells, blood-derived samples, cultured cells, and tissues.
• Execute single-cell partitioning, GEM generation, barcoding, cDNA synthesis, amplification, and NGS library preparation using platforms such as 10x Genomics Chromium.
• Prepare libraries for gene expression and multiomic applications, including CITE‑seq, immune profiling, chromatin accessibility, and spatial transcriptomics.
• Perform library quality control using instruments such as Bioanalyzer, Tape Station, Fragment Analyzer, Qubit, and qPCR.
• Prepare sequencing-ready libraries, coordinate sequencing submissions, and maintain accurate sample metadata and experimental records.
• Collaborate with scientists and bioinformaticians on experimental design, sample handling, sequencing depth, multiplexing strategies, and appropriate controls.
• Troubleshoot assay failures, conduct root‑cause investigations, and optimize protocols to improve sample quality, reproducibility, and efficiency.
• Contribute to SOP development, assay qualification, reagent management, instrument maintenance, and laboratory readiness.
• Communicate experimental results and technical recommendations while providing guidance to junior team members as appropriate.

• Bachelor's degree in Molecular Biology, Cell Biology, Genomics, Biotechnology, or a related scientific discipline with 5+ years of relevant experience; OR Master's degree with 2+ years of relevant experience.
• Hands‑on experience with Next‑Generation Sequencing (NGS) library preparation, preferably single‑cell RNA sequencing.
• Practical experience with 10x Genomics Chromium or comparable single‑cell sequencing platforms.
• Strong understanding of RNA handling, molecular biology techniques, and single‑cell sample preparation.
• Experience working with primary cells and/or tissue samples, including cell viability assessment and preparation of high‑quality cell suspensions.
• Proficiency in library quality control, experimental troubleshooting, and laboratory documentation.
• Ability to independently manage multiple experiments in a fast‑paced research environment.

• Experience with multiomic single‑cell applications such as CITE‑seq, scATAC‑seq, immune repertoire profiling, cell hashing, or feature barcoding.
• Familiarity with spatial transcriptomics and downstream sequencing quality metrics.
• Experience with laboratory automation or liquid‑handling platforms.
• Knowledge of GxP, regulated documentation practices, biomarker research, or translational research environments.
• Experience developing SOPs, optimizing workflows, and mentoring junior team members.

• Fully onsite laboratory position in Boston, Massachusetts.
• Requires regular hands‑on work with biological specimens, molecular biology reagents, and laboratory instrumentation.
• Occasional flexibility may be required to support time‑sensitive sample processing and experimental schedules.

• Work with advanced single-cell sequencing technologies.
• Contribute to clinical research and molecular biology development programs.
• Participate in experimental design, protocol optimization, and workflow improvements.
• Collaborate with multidisciplinary scientific teams and expand expertise in emerging genomics technologies.