Senior Bacteriology Technician

Join to apply for the Senior Bacteriology Technician role at Alaka`ina Foundation Family of Companies

5 days ago Be among the first 25 applicants

Join to apply for the Senior Bacteriology Technician role at Alaka`ina Foundation Family of Companies

Get AI-powered advice on this job and more exclusive features.

The Alaka`ina Foundation Family of Companies (FOCs) has a Potential need for a Senior Bacteriology Technician to support our government customer located in Fort Detrick, Frederick, MD.

DESCRIPTION OF RESPONSIBILITIES:

• Assist PI in Molecular Microbiological Experiments aimed at defining elements and factors involved in bacterial virulence. Assist in approximately 2 animal protocols conducted by other investigators.
• Complete approximately 25 Opsonic Adherence assays to test the efficacy of animal sera (Rabbit and NHP) in the presence of Baby Rabbit Complement to adhere Ames bacilli and spores to RAW 264.7 macrophage cells.
• Complete over 1000 ELISAs (including retesting of samples where needed):
  Yersinia pestis novel vaccine strategies
• Burkholderia novel vaccine strategies
• Yersinia pestis novel mAb identification
• Burkholderia novel mAb identification
• Francisella tularensis novel vaccine strategies/animal modeling
• Coxiella burnetii novel vaccine strategies/animal modeling
• Prepare approximately 10 different Reagents / Media for the lab (including R Medium, DMEM/FBS, LB Broth, Sodium Bicarbonate, APS LB Agar, BHI, etc).
• Plate approximately 100 LB plates for use for Ames sporulation isolation and purification.
• Isolate and purify approximately 10 plasmid DNA specimens.
• FITC label and count approximately 10 Ames samples to check ratio of Bacilli:
  Spores over multiple time points.
• Order lab supplies for BSL-2 and BSL-3 laboratories including common supplies, custom orders, and equipment.
• Present overview of data collected from Opsonic Adherence and ELISA at group meeting; present progress reports to supervising investigator. Use computer to prepare graphs and write reports for supervising investigator.
• Perform approximately 100 Immunological, antibody determinations.
• Perform approximately 100 ELISAs on mouse, nonhuman primate, and/or human serum.
• Perform approximately 50 Cytokine analyses. Measures cytokines in mouse, nonhuman primate, and/or human serum. Measures cytokines in cell cultures and cell extracts. Uses a Luminex instrument for analysis.
• Perform approximately 50 Flow Cytometry tasks, determining cellular distribution in mouse samples and intracellular cytokine expression. Uses a BD FACS Calibur and BD FACS Canto II to analyze cellular distribution.
• Perform approximately 25 Cell Biology/Biochemical/microbiological tasks. Performs laboratory tests, such as protein gel electrophoresis, western blot analysis, growth of macrophage cell lines, live-dead cell determinations, prepares microbiological media for bacterial cultures.
• Organize and maintain data generated from immunological and microbiological studies.
• Responsible for managing approximately 80 hand receipt items and maintaining laboratory equipment.
• Complete approximately 10 anthrax toxin neutralization (TNA) assays per year.
• Perform approximately 15 animal challenges per year and approximately 10 bacterial challenge preparations to include planning and performing complex pathogenesis studies.
• Perform approximately 1,200 animal injections per year and approximately 2,500 animal observations per year.
• Complete approximately 500 bacterial burdens in organ tissue per year.
• Perform approximately 100 Luminex Cytokine Analyses per year.
• Perform approximately 75 Macrophage phagocytosis assays per year.
• Process approximately 200 ELISAs per year.
• Perform approximately 125 Animal Necropsies per year.
• Process approximately 150 animal gavage and 250 animal blood collections per year.
• Complete approximately 40 DNA chromosomal preparations, 500 PCR reactions; and 35 Agar gel analysis per year.
• Prepare approximately 20 cloning plasmids, approximately 50 sequencing reactions, and approximately 90 plasmid preparations.
• Complete approximately 100 media preparations per year.
• Perform approximately 250 BSL-3 live/dead assays per year.
• Process approximately 40 protein and western gels per year.
• Place approximately 50 GFEBs orders per year for supplies and equipment.
• Serve as a training representative for BSL-2 laboratory skills.
• Assist PI with Project Planning, quarterly.
• Participate in training new personnel on laboratory tasks monthly.
• Perform BSAT Inventory Management weekly.
• Conduct Equipment Inventory Management twice a year in BSL-2 and BSL-3.
• Perform monthly Laboratory Management to include safety functions and chemical inventory.
• Assist the BSL-3 suite supervisor with suite supervisory duties.
• Interface with inspectors and perform monthly inspections.
• Responsible for planning and performing animal experiments under BSL-3 conditions.
• Review relevant literature monthly. May assist in the preparation standard operating procedures and manuscripts relating to their research efforts. May be expected to present…