Senior Biomarker Technician

The Alaka`ina Foundation Family of Companies (FOCs) has a potential need for a Senior Biomarker Technician support our government customer located in Fort Detrick, Frederick, MD.

DESCRIPTION OF RESPONSIBILITIES:

• Assist in the development of and independently execute protocols adhering to the experimental design for biomarker verification as determined in collaboration with the respective project Lead/PI in a BSL-2, BSL-3, and BSL-4 environment.
• Perform experiments at BSL-2, BSL-3, and BSL-4 as required by the experimental design and respective organisms.
• Perform next generation sequencing, Nanostring immunological and inflammatory panels, Luminex-based platforms, miRNA quantification, as well as other molecular diagnostic techniques.
• Conduct experiments for detection of etiologic agents within animal and clinical samples.
• Perform laboratory work requiring expertise in small animal handling with associative trainings completed.
• Assist the Lead/PIs in preparation of technical reports and manuscripts for publication
• Perform laboratory work required to fulfil experimental designs for novel sample collection/concentration/processing to include Nanotrap Concentration, and DNA/mRNA purification from both NHP and Human clinical matrices.
• Present protocols and data generated at weekly meetings.
• Perform required lab work necessary to fulfil the experimental design for biomarker verification as determined in collaboration with the respective project Lead/PI in a BSL-2, BSL-3, and/or BSL-4 environment.
• CONUS/OCONUS travel may be required in support of the diagnostic mission and/or presentation of scientific research.
• Perform sample processing techniques to include: QIAgen EZ1, MagNA Pure, and manual column-based techniques as well as novel molecular enrichment protocols for next generation sequencing and real-time PCR
• Develop and independently perform highly technical next-generation sequencing protocols for analytical, mock clinical, and/or clinical sample testing.
• Perform highly detailed clinical sample testing in support of priority BSL-2, BSL-3, and/or BSL-4 animal studies or human clinical sample surveillance studies.
• Train new laboratory members in BSL-2, BSL-3, and/or BSL-4 project-specific laboratory techniques and methods.
• Consolidate and analyze biomarker data in support of technical reports, presentations, and manuscripts.
• Facilitate the planning, preparation, and laboratory research execution support of approximately 5-8 projects per year.
• Contribute to a Biomarker Quarter and Annual Reports for associate projects.
• Contribute to the collection, management, and analysis of Biomarker Data for assigned projects.
• Author, review and revise Biomarker SOPs as required to support assigned projects.
• Other duties as assigned by Supervisor.

REQUIRED DEGREE/EDUCATION/CERTIFICATION:

Must hold Associate degree or equivalent training and expertise in small animal handling.

REQUIRED SKILLS AND EXPERIENCE:

• Must have two (2) to three (3) years’ experience supporting pre-clinical and clinical laboratory environments.
• Must have two (2) to three (3) years’ experience supporting the collection, management, and analysis of Biomarker Data.
• Must have two (2) to three (3) years’ experience supporting the development of and independently executing protocols adhering to the experimental design for biomarker verification in a BSL-2, BSL-3 environment. BSL-4 environment preferred.
• Must have one (1) to two (2) years’ experience training new laboratory members in BSL-2, BSL-3, and/or BSL-4 environments.
• Must have two (2) to three (3) years’ experience performing next generation sequencing, Nanostring immunological and inflammatory panels, Luminex-based platforms, miRNA quantification, as well as other molecular diagnostic techniques.
• Direct or Indirect experience performing novel sample collection/concentration/processing to include Nanotrap Concentration, and DNA/mRNA purification from both NHP and Human clinical matrices.
• Direct experience performing sample processing techniques to include: QIAgen EZ1, MagNA Pure, and manual column-based techniques as well as novel molecular enrichment protocols for next generation sequencing and real-time PCR.

REQUI…