Senior Bacteriology Technician B

Cape Fox subsidiary Eagle Health, LLC is accepting resumes for a
SENIOR BACTERIOLOGY TECHNICIAN
to support the members rick, Maryland.This position is contingent upon contract award.

Incumbents are encouraged to apply!

The salary range (or hiring range) for this exempt position has been established at:$85,000-$95,000
.

The above salary range represents the company's good faith and reasonable estimate of the range of possible compensation at the time of posting. In addition, we offer a variety of benefits including company holidays, paid time off, health insurance, dental insurance, vision insurance, life and disability insurance, tuition reimbursement, as well as 401K with company match. This job will be posted until filled or withdrawn.

• Assist the Lead/PI in Molecular Microbiological Experiments aimed at defining elements and factors involved in bacterial virulence. Assist in approximately 2 animal protocols conducted by other investigators.
• Complete approximately 25 Opsonic Adherence assays to test the efficacy of animal sera (Rabbit and NHP) in the presence of Baby Rabbit Complement to adhere Ames bacilli and spores to RAW 264.7 macrophage cells.
• Complete over 1000 ELISAs (including retesting of samples where needed):
  • Yersinia pestis novel vaccine strategies
  • Burkholderia novel vaccine strategies
  • Yersinia pestis novel mAb identification
  • Burkholderia novel mAb identification
  • Francisella tularensis novel vaccine strategies/animal modeling
  • Coxiella burnetii novel vaccine strategies/animal modeling
• Prepare approximately 10 different Reagents / Media for the lab (including R Medium, DMEM/FBS, LB Broth, Sodium Bicarbonate, APS LB Agar, BHI, etc).
• Shall plate approximately 100 LB plates for use for Ames sporulation isolation and purification.
• Shall isolate and purify approximately 10 plasmid DNA specimens.
• FITC label and count approximately 10 Ames samples to check ratio of Bacilli:
  Spores over multiple time points.
• Order lab supplies for BSL-2 and BSL-3 laboratories including common supplies, custom orders, and equipment - approximately 400 items ordered along with 5 pieces of equipment.
• Present overview of data collected from Opsonic Adherence and ELISA at group meeting; present progress reports to supervising investigator. Use computer to prepare graphs and write reports for supervising investigator.
• Perform approximately 100 Immunological, antibody determinations.
• Perform approximately 100 ELISAs on mouse, nonhuman primate, and/or human serum.
• Perform approximately 50 Cytokine analyses. Measures cytokines in mouse, nonhuman primate, and/or human serum. Measures cytokines in cell cultures and cell extracts. Uses a Luminex instrument for analysis.
• Perform approximately 50 Flow Cytometry tasks, determining cellular distribution in mouse samples and intracellular cytokine expression. Uses a BD FACS Calibur and BD FACS Canto II to analyze cellular distribution.
• Perform approximately 25 Cell Biology/Biochemical/microbiological tasks. Performs laboratory tests, such as protein gel electrophoresis, western blot analysis, growth of macrophage cell lines, live-dead cell determinations, prepares microbiological media for bacterial cultures.
• Organize and maintain data generated from immunological and microbiological studies.
• Shall be responsible for managing approximately 80 hand receipt items and maintaining laboratory equipment.
• Complete approximately 10 anthrax toxin neutralization (TNA) assays per year.
• Perform approximately 15 animal challenges per year and approximately 10 bacterial challenge preparations to include planning and performing complex pathogenesis studies.
• Perform approximately 1,200 animal injections per year and approximately 2,500 animal observations per year.
• Shall complete approximately 500 bacterial burdens in organ tissue per year.
• Perform approximately 100 Luminex Cytokine Analyses per year.
• Perform approximately 75 Macrophage phagocytosis assays per year.
• Process approximately 200 ELISAs per year.
• Perform approximately 125 Animal Necropsies per year.
• Process approximately 150 animal gavage and 250 animal blood collections per year.
• Shall complete approximately 40 DNA chromosomal preparations,…