Technical Assistant, Healthcare

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The mission of the Berlin Institute of Health at Charité (BIH) is medical translation: transferring biomedical research findings into novel approaches to personalized prediction, prevention, diagnostics and therapies and, conversely, using clinical observations to develop new research ideas. The aim is to deliver relevant medical benefits to patients and the population ce 2021 the BIH has been integrated into Charité as its so‑called third pillar.

For the team of Prof. L. Vallier starting now until 25 September 2028 we are looking for a Technical Assistant (d/f/m) in full‑time (38.5 h/week). The Vallier lab based at the BCRT will take advantage of human induced pluripotent stem cells and primary organoids to understand liver development in humans and to generate cells with a clinical interest for modelling diseases and for cell‑based therapies applications.

More precisely, we investigate the molecular mechanisms controlling cell fate decisions during human development and exploit the resulting knowledge to produce liver cells especially hepatocytes and cholangiocytes.

The candidate will join the ARPA‑H project LIVE which aims to bioprint an entire human liver for transplantation in the context of acute liver disease. The role of the candidate will be (i) to identify and to characterise GMP‑grade human induced pluripotent stem cell lines suitable for the project, (ii) to produce, to characterise and to quality‑control large quantities of liver cells for the project, and (iii) to develop new methods for differentiation of hiPSC lines using bioreactor technology.

The resulting cells will then be used to bioprint an artificial liver in vitro in collaboration with the international network of the ARPA‑H LIVE program.

• Cell culture production (grow, characterise, differentiating human pluripotent stem cells)
• Application of previously determined analytical procedures
• Filling, operation and calibration/programming of the instruments to be used
• Evaluation and interpretation of the analysis results with regard to plausibility and quality
• Receipt, documentation and tracking of valuable samples
• Review and qualitative evaluation of the sample
• Technical elaboration of the experimental setup
• Maintenance of laboratory equipment, if necessary organisation of external service and repair measures
• Supervision of general laboratory operations and organisation
• Maintenance of the laboratory workplace, taking into account occupational health and safety and disposal guidelines
• Ordering and stocking of consumables, media and solutions
• Performance of analytical procedures (application of previously determined analytical procedures, filling, operation and calibration/programming of the instruments to be used, evaluation and interpretation of the analysis results with regard to plausibility and quality)

• Bachelor or equivalent in cell biology, bioengineering or regenerative medicine.
• Experience in tissue culture especially with human pluripotent stem cells.
• Basic experience in flow cytometry, qPCR, and confocal imaging.
• Experience in working independently while keeping a strong team spirit.
• Strong self‑motivation, organisational skills, and ability to lead and develop scientific projects.
• Scientific curiosity, analytical thinking, and interest in contributing to projects.
• Ability to work in a team of young scientists, to be flexible, and flourish in a fast‑paced environment.
• Strong communication and interpersonal skills, ability to work collaboratively effectively with individuals of different backgrounds in a multidisciplinary team, team orientated with excellent organisational skills including project management and good record keeping important.
• Fluent English.

• A varied job in a forward‑looking research institute
• Pay group E9b TVöD VKA‑K. The classification is based on qualifications, the respective experience level is calculated on the basis of professional experience. The annual salary (gross) is stated for a full‑time position without special or additional payments. The collective agreement can be…