Research assistant in CRISPR‑engineered cellular models of genetic disorders

Location: Candiolo

IIGM is located at the FPO IRCCS Candiolo Cancer Institute, Candiolo Turin - Italy. The main goal of FPO IRCCS Candiolo and IIGM is to foster and develop excellence in research and education, with special reference to cancer biology and cancer biomarker discovery. The institute provides high-level scientific education for students and post-doctoral fellows, and access to state-of-the‑art research facilities and international networks.
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Posizione
The Chemical Biology Lab at the Italian Institute for Genomic Medicine (IIGM) is seeking highly motivated candidates at all levels of seniority (predoc, postdoc) to work in high‑content imaging and analysis with a focus on cell‑to‑cell heterogeneity and phenotypic variability in membrane trafficking. The laboratory investigates key aspects of Rab GTPases and their involvement in hereditary genetic diseases. The project aims to generate cellular model of steroid‑resistant nephrotic syndrome (SRNS) disease and perform genotype‑phenotype correlation analysis using molecular and computational tools developed in the lab.

By integrating phenotypic mapping with biochemical and genetic information, we seek to elucidate the biological functions of rare genetic variants causing steroid‑resistant nephrotic syndrome. The Institute provides state‑of‑the‑art genomics, single‑cell genomics sequencing platforms and high‑content imaging platforms.
Responsibilities

Generate isogenic HK-2 cell lines harbouring genetic mutations causing SRNS disease
Perform immunostaining of Rab GTPase and other proteins involved in intracellular trafficking
Perform biochemical experiments on Rab GTPase regulators
Conduct image acquisition using automated microscopy systems
Perform image data analysis of high‑dimensional dataset
Contribute to ongoing research group activities and collaborative projects

Qualifications and Requirements

Bachelor’s degree in biotechnology, biology, medicine, chemical biology, pharmacy or a related field
1 year experience in at least one of the following subjects:

generation of knock‑in CRISPR/Cas9 edited human cells
protein purification
high‑content imaging and data analysis
mammalian tissue culture (cells and/or organoids)

Good command of the English language
Knowledge of Italian is an advantage, but not strictly necessary

Soft Skills

Curiosity and active learning
Critical thinking and problem-solving
Time management
Adaptability and flexibility
Effective communication in cross‑cultural environments

Positions
No. 01
Offered contract
Coordinated and Continuous Collaboration contract () on a project basis duration to be defined. Competitive remuneration based on the candidate’s personal qualifications.
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