Research Laboratory Technician

Research Laboratory Technician

Biomedical & Life Sciences

We are looking for a Research Technician to support our research studies exploring how a ubiquitin ligase contributes to DNA replication stress and repair. The position is funded by an Academy of Medical Sciences grant awarded to Dr. Panagiotis Kotsantis, and is available for 24 months.

• Generate and characterise engineered cell lines with inducible gene expression, gene depletion, and targeted genetic modifications, including domain deletions and site‑directed mutations.
• Investigate cellular responses to DNA damage and replication stress using a range of molecular and cell biology approaches, including immunofluorescence microscopy, immunoblotting, DNA fibre assays, and micronucleus analysis.
• Examine protein localisation and chromatin‑associated dynamics at sites of DNA replication and genome maintenance using advanced imaging and proteomic techniques.
• Characterise protein–protein and protein–nucleic acid interactions through biochemical and proximity‑based assays, including immunoprecipitation and proximity ligation assays.
• Identify and analyse interaction networks and candidate substrates of a target pathway regulator using mass spectrometry‑based proteomics.
• Prepare and conduct experiments including engineered cell line generation, DNA damage response analysis, protein interaction and localisation studies, and proteomic identification of molecular interaction networks.
• Data acquisition and management, and contributing to analysis and reporting.
• Support day‑to‑day lab operations, including equipment maintenance, ordering supplies, record keeping, and assisting with regulatory documentation.
• Provide technical training to students and other lab members.

• Bachelor’s degree in biomedicine, biomedical sciences, biochemistry or a related discipline.
• Experience supporting biomedical studies, including mammalian cell culture, immunoblotting, immunofluorescence, DNA cloning, molecular biology techniques and data analysis using Graph Pad‑Prism and Image
  
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• Strong organisational and administrative skills, including inventory management, maintaining accurate records, and report writing.
• A proactive approach to problem‑solving and technical troubleshooting, with the confidence to take initiative and work independently.
• Excellent written and verbal communication skills, and a collaborative, team‑oriented approach.
• Experience with the DNA fibre assay is desirable but not essential; training will be provided for the right candidate.

Most importantly, you will bring motivation, attention to detail, and enthusiasm for contributing to meaningful interdisciplinary research.

We warmly welcome applicants from all sections of the community regardless of their age, religion, gender identity or expression, race, disability or sexual orientation, and are committed to promoting diversity and equality of opportunity.

Please contact Dr Panagiotis Kotsantis at