Molecular Cloning & Protein Engineering Scientist

You will be responsible for engineering and building therapeutic constructs. This is a hands‑on, high throughput role where you will design, build and iterate on novel CRISPR‑Based constructs that enable programmable, target‑selective approach across multiple oncology indications. Your work will directly shape the therapeutic candidates that enter preclinical development.

• Design, engineer, and optimize Cas‑based therapeutic constructs, including conditional and programmable protein architectures.
• Engineer proteins for enhanced specificity, activity, and control using rational design, structure‑guided engineering, and directed evolution.
• Expand the modularity of the platform by developing constructs targeting additional disease‑relevant genes.
• Design, clone, and validate guide RNA libraries for specificity and off‑target profiling assays (e.g., ONE‑seq, CHANGE‑seq, or related technologies).
• Design, execute, and analyze rapid design‑build‑test cycles to improve construct performance.
• Collaborate across molecular biology, protein engineering, computational biology, and translational research teams to advance therapeutic programs.

• PhD in Synthetic Biology
• At least 5 years of hands‑on experience in molecular cloning using techniques such as Gibson Assembly, Golden Gate Assembly, and Gateway cloning.
• At least 5 years experience of Protein engineering (engineered the function, not just been a part)
• Extensive experience designing and engineering CRISPR/Cas constructs that enable programmable, target‑selective approach across multiple oncology indications, including development of novel protein functions through directed evolution or rational engineering.
• Strong expertise in mammalian and bacterial expression vector design, construct optimization, and stable cell line generation.
• Demonstrated ability to independently execute rapid iterative construct design and optimization.
• Experience developing and utilizing fluorescent and/or luminescent reporter assay systems.
• Strong structural biology and wet‑lab expertise, with experience applying structural insights to protein engineering challenges.
• Demonstrated ownership of engineering protein function, rather than solely supporting execution of established workflows.
• Computational experience supporting protein or CRISPR engineering, including sequence analysis, structural modeling, guide RNA design, scripting, or bioinformatics.

• Experience with inducible or conditional protein systems.
• Familiarity with base editing, prime editing, or other next‑generation genome editing technologies.
• Experience with NGS library preparation and sequencing‑based screening workflows.
• Background in structural biology, protein biochemistry, or computational protein engineering.
• Experience working in fast‑paced biotechnology or therapeutic discovery environments.