Technician, Gene and Cell Therapy – Translational Research

• Conduct microbiology workflows to prepare plasmid stocks and libraries
• Perform routine cloning to generate plasmid and lentiviral vector constructs
• Conduct cell culture and transfection workflows for lentiviral vector production
• Determine viral particle titers and perform quality control using flow cytometry and droplet digital PCR
• Test and optimize viral particle production and quality-control protocols
• Organize and track samples during production and storage
• Support laboratory operations, including inventory management, solution preparation, routine laboratory tasks, and mycoplasma testing
• Contribute to preparing, reviewing, and completing study reports and protocol SOPs
• Contribute to research team and department activities, laboratory duties, and group and department meetings
• Work closely with the Translational Research Team to plan and conduct routine lentiviral production runs and maintain laboratory documentation and reports

• Candidates must have experience in cellular and molecular biology
• Experience of working with Lentiviral Vectors
• Skills in mammalian cell culture, vector engineering, genetic engineering, cloning, PCR, transfection, transduction, and assay development
• Organized and proactive, with the ability to manage multiple tasks efficiently and remain flexible and adaptable
• Strong interpersonal and communication skills for collaboration with the research team and cross-functionally
• Keen attention to detail and good analytical ability to interpret data sets
• Ability to analyze and write up data and maintain accurate records
• IT skills including Word, Excel, and Power Point
• Experience working with an ELN such as Benchling preferred
• Highly motivated and eager to take responsibility for contributing to Orchard's mission
• BSc or MSc with a focus on a relevant biological discipline, including molecular biology or biotechnology, plus at least 2 years of laboratory work with viral vectors and molecular biology; or more than 5 years of experience in virology, lentiviral vector production, and genetic engineering technologies
• Participation in E-Verify

Demonstrates expertise in microbiology workflows, including plasmid and lentiviral vector production, alongside strong skills in cellular and molecular biology. Proficient in laboratory operations, data analysis, and maintaining accurate records while collaborating effectively with research teams.

• Lentiviral Vector Production
• Cell Culture
• Cloning
• PCR
• Genetic Engineering

• Mammalian Cell Culture
• Vector Engineering
• Transfection
• Transduction
• Assay Development
• Quality Control
• Flow Cytometry
• Droplet Digital PCR
• Data Analysis
• Laboratory Documentation

• Organized
• Proactive
• Strong Interpersonal Skills
• Attention to Detail
• Analytical Ability

• Microbiology
• Molecular Biology
• Biotechnology
• Viral Vectors
• E-Verify

• ELN (Benchling)
• Word
• Excel
• Power Point