Research Specialist C​/D

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Research Specialist C/D

Research Specialist C

The Research Specialist C/D position in The Center for Cellular Immunotherapies will support research efforts in CAR T-cell therapy development by performing complex in vitro and in vivo experiments.

• Prepare cell culture materials, media, and reagents.
• Follow established protocols and maintain accurate experimental records.
• Isolate, transduce, transfect, electroporate, expand, formulate, and cryopreserve human T lymphocytes. Perform in vitro experiments to assess T cell phenotype and function (flow cytometry and cocultuure assays).
• Perform molecular assays, optimize experimental conditions.
• Follow standardized protocols and optimize methods to establish in vivo tumor models (IP, IV, orthotopic, subcutaneous) and monitor responses to various cell therapies.
• Perform injections, tumor measurements, imaging, bleeding, necropsy, and tissue harvesting.

• Maintain organized, real-time records of experiments for traceability and reproducibility.
• Keep accurate data records into laboratory notebooks and group folders and provide data as needed.
• Present data to supervisor and in small group meetings.

• Maintain laboratory organization and inventory.
• Collaborate with team members to achieve research goals.
• Ensure compliance with ethical standards and proper handling of animals and hazardous materials.

In addition to the duties listed, the Research Specialist D will design panels and perform multiparametric flow cytometry for phenotypic characterization of engineered T cells and co-cultures; analyzes datasets using appropriate software. Design, plan and carry out molecular assays. Independently develop protocols, ensure data collected is verified, complete and consistent and properly recorded in digital folders and lab notebook. Provides data summaries, tables, graphs and figures for reports and presentations.

Train students and new members on general laboratory procedures, providing advice and mentorship on technical procedures.

• Bachelor of Science and 3 to 5 years of experience or equivalent combination of education and experience is required.
• Demonstrated proficiency in tissue culture techniques and basic molecular biology methods.
• Extensive in vivo experience with mouse models, including NSG/syngeneic tumor modeling, intravenous (IV),
• Ability to follow established protocols accurately and maintain detailed records.
• Strong organizational skills and ability to work collaboratively in a team environment

• Experience with molecular biology techniques,…