Scientist- Abraham's Lab

Join our Team! The Abraham Laboratory in the Department of Computational Biology e Children's Research Hospital is seeking an innovative and collaborative Scientist to investigate how gene regulation and nuclear organization shape mammalian cell identity and contribute to disease, including pediatric cancers.

This is an exciting bench-based role for a hands-on experimental scientist who is passionate about uncovering the mechanisms that control gene expression.

The successful candidate will study cis-and trans-regulatory mechanisms using cutting-edge molecular biology technologies and independently design and execute high-throughput functional genomics assays.

Working at the intersection of molecular biology, functional genomics, and cancer biology, you will design and execute cutting-edge experiments that advance our understanding of how regulatory elements such as enhancers and super-enhancers drive normal development and disease.

As a key member of a highly collaborative multidisciplinary team, you will lead and contribute to research projects utilizing advanced genomic technologies, cell-based systems, and imaging approaches to answer fundamental biological questions with direct relevance to human health.

The ideal candidate is a highly independent, hands-on scientist with strong troubleshooting skills and a collaborative mindset. This individual will serve as a key experimental partner within a shared-resource environment, working across multiple research projects focused on gene regulation and functional genomics.

Candidates are not required to have expertise in every area listed. We welcome individuals with experience in a combination of these techniques who are eager to expand their skill set and contribute across diverse research projects.

Molecular & Cellular Biology

• Molecular cloning
• Cell culture and tissue culture
• Viral transduction and cell line engineering
• Tissue processing and sample handling

• Strong hands-on experience with at least one high-throughput sequencing-based assay such as:
  • ChIP-seq
  • CUT&RUN
  • ATAC-seq
  • RNA-seq
  • Hi-C or related genomic technologies
• Ability to independently troubleshoot and optimize genomics workflows

• Immunofluorescence staining
• DNA and RNA FISH
• Confocal microscopy
• Cellular imaging-based assays

• Massively Parallel Reporter Assays (MPRA)
• High-throughput functional genomic screening
• Research involving transcriptional condensates, enhancer biology, or gene regulatory mechanisms
• Experience generating and analyzing large-scale genomic datasets

Depending on their expertise and project needs, the successful candidate may contribute to initiatives involving:

• MPRA-based functional genomics studies
• DNA and RNA imaging assays
• Transcriptional condensate biology
• Enhancer and super-enhancer regulation
• Genome-wide sequencing and functional genomics applications
• Large-scale dataset generation and characterization efforts

• Coordinate multiple research projects, carry out bench laboratory experiments utilizing various techniques, and determine whether additional or other tools, methods, or data are required
• Provide input regarding research protocols and experiments. Sets up protocols and assists in developing new laboratory techniques as required.
• Monitor the quality and integrity of recorded information and data.
• Identify, process, organize, interpret, review, and faithfully, comprehensively, and durably report relevant data.
• Draft sections of research manuscripts.
• Provide basic lab operation support to include equipment maintenance and purchasing. Monitor inventory levels and order lab supplies as needed.
• Collaborate on project and analysis design under the guidance of multiple invested PIs.
• Set personal task-level priorities across multiple interconnected projects.
• Adhere to field and lab standards for data generation and analysis.
• Perform other duties as assigned to meet the goals and objectives of the department and institution.
• Maintain regular and predictable attendance, especially in-person attendance at meetings and relevant presentations.
• Increase in independence and expertise over time.

• Ability to rapidly learn, optimize, and implement new or advanced techniques as required by the evolving research direction.
• Experience leading or substantially driving complex…