Part-Time Laboratory Technician

St. John's University

Salary: $22.00 - $25.00 Hourly

Job Type: Part-Time Staff

Job Number:

Location:

Queens Campus, NY

St. John's, established in 1870, has two New York City campuses;international locations in Rome, Italy;
Limerick, Ireland; and Paris, France; and study abroad locations around the world. The Princeton Review and other top rankings consistently recognize the University's outstanding academics, diverse student body, dynamicinternship, and volunteer opportunities, focus on student life, and diverse study abroad offerings. St. John's University offers morethan 100 undergraduate and graduate programs in its six colleges and schools, with a growing number of programs offered online.

The University is accredited by the Middle States Commission on Higher Education and 12 other major academic and professional associations.

A dedication to diversity, equity and inclusion is at the heart ofour mission. As a Catholic and Vincentian university, St. John's is committed to institutionalizing practices of inclusive excellence to ensure that we welcome and celebrate the intrinsic worth of all members of our community. We will become an even strongeruniversity as we enhance equity at every level of our institution.

Our graduates will excel in the competencies and valuesneeded for leadership and service in a rapidly evolvingworld.

This part-time laboratory technician position supports an NIHR
16-funded research program in the Department of Biological Sciences. The primary responsibility of this position is to perform

Western blotting and related cell and molecular biology experiments and to generate high-quality, reproducible experimental data.

The ideal candidate will have substantial hands‑on experience with Western blotting and should be able to perform the techniqueindependently, including sample preparation, SDS-PAGE, proteintransfer, antibody incubation, imaging, data analysis, and troubleshooting. Experience with mammalian cell culture and othermolecular and cellular biology techniques is also desirable.

The technician will work closely with faculty and students and mayassist in establishing standardized laboratory procedures and training laboratory members in Western blotting and relatedtechniques. This position has no supervisory or leadership responsibilities

• Independently perform Western blotting experiments, includingprotein sample preparation, SDS-PAGE, membrane transfer, blocking,primary and secondary antibody incubation, washing, imaging, and quantitative analysis.
• Optimize and troubleshoot Western blotting conditions to obtainreproducible, publication-quality data.
• Maintain detailed experimental records, including sample information, experimental conditions, raw images, and quantitativeresults.
• Perform mammalian cell culture and prepare cell lysates forbiochemical analysis.
• Assist with routine molecular and cellular biology experiments related to the NIH-funded research project.
• Establish and follow standardized Western blotting procedures to improve experimental reproducibility within the laboratory.
• Assist faculty and students with Western blotting techniques and troubleshooting as needed.
• Prepare laboratory reagents, buffers, and stocks required for Western blotting and related experiments.
• Maintain and appropriately use laboratory equipment related toprotein analysis and cell culture.
• Follow University laboratory safety, biosafety, and research compliance requirements.
• Communicate experimental progress, technical problems, and results clearly with the Principal Investigator and other research team members.

Required

Education and Experience:

• Bachelor's degree or an equivalent combination of education…