UNIV - Research Specialist - Regenerative Medicine & Cell Biology

The research specialist I will work under general supervision by the PI and Lab manager and will be part of a basic science research laboratory that focuses on mitochondria biology and treatment of mitochondrial diseases, especially Charcot-Marie-Tooth disease type 2A (CMT2A). The work of the individual will be with emphasis on cell culture, including maintaining cell lines including thawing, expansion, passaging using strict aseptic techniques and performing independent experiments including staining imaging and evaluation.

Furthermore, the individual will be involved in the maintenance of the transgenic mouse and rat colonies, histology and immunohistochemistry, microscope imaging, animal behavioral and electrophysiological studies, and utilizing various other laboratory techniques including PCR, western blotting.

Medical University of South Carolina (MUSC - Univ) Worker Type Employee Worker Sub-Type Research Grant Cost Center CC001060 COM REG MED Administration CC Pay Rate Type Hourly Pay Grade University-GEN
06 Pay Range 37,200.

- 59,500.000 Scheduled Weekly Hours 40 Work Shift

The research specialist I will work under general supervision by the PI and Lab manager and will be part of a basic science research laboratory that focuses on mitochondria biology and treatment of mitochondrial diseases, especially Charcot-Marie-Tooth disease type 2A (CMT2A). The work of the individual will be with emphasis on cell culture, including maintaining cell lines including thawing, expansion, passaging using strict aseptic techniques and performing independent experiments including staining imaging and evaluation.

Furthermore, the individual will be involved in the maintenance of the transgenic mouse and rat colonies, histology and immunohistochemistry, microscope imaging, animal behavioral and electrophysiological studies, and utilizing various other laboratory techniques including PCR, western blotting.

A high school diploma and two years work experience in a clinical or research setting; a bachelor's degree in chemistry, biology, or other natural, life or health care science may be substituted for the two years of required experience.

Bachelor’s degree in biology, chemistry, biochemistry, molecular biology or other life science is required. Strong organizational, record keeping, and interpersonal communication skills are required. Previous laboratory experience with cell culture and sterile techniques is preferred but not required. Preference will be given to candidates with willingness to learn new techniques and adapt to evolving project needs. Must be able to work with animals (mouse and rat).

Employee is supervised by the PI and lab manager but expected to conduct experiments, collect and record data with decent level of independence and discretion.

Work is conducted in a basic biomedical laboratory that focuses on mitochondrial diseases and their treatment.

Perform mammalian cell culture, ex vivo tissue culture experiments, with a range of experimental assays. Percent of Total Time: 35 - Essential Perform simple to complex laboratory procedures in one or more areas of laboratory investigation such as: DNA isolation and PCR; western blot; histological sample preparation and staining, immunohistochemistry, microscopy and a variety of other molecular biology procedures. Percent of Total Time: 35 - Essential Work closely with the PI and lab manager for animal colony management, including genotyping of transgenic mouse and rat lines, with assistance in behavioral and electrophysiological experiments including drug…