UNIV - Post Doctoral Scholar - Pediatrics: DCRI Job Town of Charleston New York USA,Research/Development

Job Description Summary A cancer research laboratory under Dr. Jezabel Rodriguez-Blanco within the Hollings Cancer Center and the Darby Children’s Research Institute has an opening for a postdoctoral position. Entity Medical University of South Carolina (MUSC - Univ) Worker Type Employee Worker Sub-Type Research Grant Cost Center CC001013 COM PEDS Children's Research Institute CC Pay Rate Type Hourly, Salary Pay Grade University-00 Pay Range 0.00 - 0.00 - 0.000 Scheduled Weekly Hours 40 Work Shift Job Description This lab utilizes in vitro, ex vivo, and in vivo models, including genetic mouse models and patient-derived orthotopic xenografts, to investigate the intracellular drivers of pediatric brain tumor growth.

The primary research focus is on elucidating the signaling mechanisms that contribute to the relapse of medulloblastoma, the most common malignant pediatric brain tumor. The research will employ a range of complementary techniques in cellular and molecular biology, including immunoblotting, RT-qPCR, immunoprecipitation, ChIP-PCR, IHC and IF imaging, flow cytometry, as well as bulk and single-cell RNA sequencing and functional cell-based assays.

The ideal candidate will be highly motivated and hold a recent Ph.D. in cancer biology, neurosciences, or a related field, with a strong background in molecular and cell biology, and biochemistry. While experience with mouse genetic models and survival surgeries, including brain tumor implants, is preferred, it is not mandatory. Familiarity with CRISPR screening and bioinformatics analyses is also considered an advantage.

Job Duties:

(Essential) 30% - Experimental Design and Execution:
Work collaboratively with the PI and other lab members to design and execute experiments using advanced mouse genetic models and cell-based studies to identify drivers of medulloblastoma growth and relapse. Responsibilities include genotyping genetically-engineered mice, maintaining animal colonies, conducting in vivo experiments such as treatment studies and orthotopic/subcutaneous tumor cell implantation, monitoring disease progression and tumor growth, performing necropsies on experimental mice, and conducting in vitro studies, including preparing cells from these models.

Lab techniques involve cell culture, molecular and cellular biology assays (e.g., cell viability, IF, flow cytometry, immunoblotting, PCR, qPCR, reporter assays, IHC, ChIP-PCR), and functional genomic assessments of gene expression and transcription factor binding to target genes, including single-cell RNA sequencing, standard RNA sequencing, and ChIP sequencing. Additional tasks include recombinant DNA work and lentiviral production for cell transfection and transduction experiments.
(Essential) 30% - Data Collection and Analysis:
Support data collection and analysis from experiments, including maintaining accurate and detailed records on a daily basis, identifying and utilizing appropriate software for data analysis, collaborating with the Biostatistics and Bioinformatics Research Shared Resources to ensure data quality, interpreting results, and planning new experiments. These responsibilities are essential.
(Essential) 30% - Manuscript and Grant Preparation:
Contribute to manuscript and grant preparation based on experimental findings. Responsibilities include keeping current with relevant literature, preparing data figures, drafting manuscripts and grant sections alongside the PI and other team members, presenting ongoing research in seminars, attending departmental seminars, and participating in journal clubs.
(Essential) 10% - Laboratory Maintenance and Compliance:
Assist with laboratory upkeep and regulatory compliance, including ordering supplies, maintaining stock of experimental reagents and solutions, ensuring equipment functionality, preparing IACUC protocols and amendments as needed, washing glassware, and ensuring overall lab cleanliness.

Required Minimum

Education and Experience:

The candidate should hold a recent Ph.D. in Neurosciences, Cancer Biology, or a related field, with strong skills in molecular and cellular biology and biochemistry. Experience working with mouse genetic models is desirable but not required. Additional experience with CRISPR screens and bioinformatic analyses is advantageous. Must have previous experience in a laboratory.

Preferred Knowledge, Skills, and Abilities:

Strong organizational, record-keeping, interpersonal, and communication skills are essential. The candidate must be…