Scientist, DNA Construct & Plasmid Map QC

Syst Immune is a leading and well‑funded clinical‑stage biopharmaceutical company located in Bothell, WA and Princeton, NJ. It specializes in developing innovative cancer treatments using its established drug development platforms, focusing on bi-specific, multi-specific antibodies, and antibody-drug conjugates (ADCs). Syst Immune has multiple assets in various stages of clinical trials for solid tumor and hematologic indications. Alongside ongoing clinical trials. Syst Immune has a robust preclinical pipeline of potential cancer therapeutics in the discover and IND-enabling stages, representing cutting‑edge biologics development.

We offer an opportunity for you to learn and grow while making significant contributions to the company's success.

We are seeking a Scientist level molecular biologist to lead DNA construct design, high-throughput cloning, and sequence quality control for our AI-driven antibody and protein discovery platform. This hands-on role will deliver sequence-verified constructs to support a range of research and expression applications, while maintaining accurate, traceable records linking each molecular design to its corresponding physical sample.

This is full-time onsite in Bothell, WA.

• Design expression constructs and primers, selecting appropriate vectors, regulatory elements, tags, and codon usage for the intended expression system.
• Execute and troubleshoot cloning, assembly, and site-directed mutagenesis using methods such as Gibson assembly, Golden Gate, restriction-ligation, and In-Fusion.
• Develop and scale plate-based cloning workflows, including bacterial transformation, colony screening, plasmid preparation, and sequence verification.
• Review Sanger and whole-plasmid sequencing data against intended designs; investigate sequence discrepancies and document construct release decisions.
• Maintain annotated, version-controlled plasmid maps and sequence records in Geneious with traceability to sample IDs and storage locations.
• Establish naming conventions, annotation standards, QC acceptance criteria, and SOPs; monitor cloning success and turnaround time.
• Partner with protein science and automation teams to transfer verified constructs into expression workflows and improve reproducibility and throughput.

• PhD in molecular biology, biochemistry, or a related field with 0-3 years of relevant industry experience.
• Strong practical expertise in DNA construct and primer design, molecular cloning, and troubleshooting, including proficiency in at least two assembly or cloning approaches.
• Proficiency in Benchling, Geneious, or comparable software for sequence analysis, alignment, plasmid annotation, and construct record management.
• Experience interpreting sequencing results, identifying sequence errors, and maintaining accurate plasmid maps and sample records.
• Experience with parallel or plate-based cloning workflows, strong attention to detail, and clear documentation and cross-functional communication.

• Experience with 96-well cloning, whole-plasmid NGS analysis, or liquid-handling automation.
• Antibody construct design across formats such as Fab, IgG, VHH, scFv, and multispecific proteins.
• Python or other scripting skills for sequence processing, QC, or workflow automation.
• Familiarity with protein purification and characterization, including ÄKTA, SEC, BLI, or SPR.

The expected base salary range for this position is $90,000 - $115,000 annually. Actual compensation will be based on a variety of factors, including but not limited to a candidate’s level qualifications, experience, and skills.

While most offers typically fall within the low to mid-point of the range, we may extend an offer toward…