Senior Scientist; Computational - Genetics

Position Summary

Scheduled

Hours:

40

The Cremins lab works at the spatial biology-technology interface to understand chromatin-to-synapse communication during neural circuit activation in the mammalian brain. We aim to understand how chromatin works through long-range physical folding mechanisms to encode neuronal specification and long-term synaptic plasticity in healthy and diseased neural circuits. We pursue a multi-disciplinary approach integrating data across biological scales in the brain, including molecular Chromosome-Conformation-Capture sequencing technologies, single-cell imaging, optogenetics, genome engineering, induced pluripotent stem cell differentiation to neurons/organoids, and in vitro and in vivo electrophysiological measurements.

Our long-term scientific goal is to dissect the fundamental mechanisms by which chromatin architecture causally governs genome function and, ultimately, long-term synaptic plasticity and neural circuit features in healthy mammalian brains as well as during the onset and progression of neurodegenerative and neurodevelopmental disease states. Our long-term mentorship goal is to develop a diverse cohort of next-generation scientists cross-trained in molecular and computational approaches.

We seek to create a positive, high-energy environment with open and honest communication to empower individuals to discover and refine their purpose and grow into the best versions of themselves.

Will be responsible for both specific research projects as well as the oversight of the computational infrastructure and computational code and data analysis standards for the laboratory. Will play a leadership role in working with those who have built an in-house computational cluster with backup servers in a second location to facilitate movement of all of the lab’s data off the cluster and onto local storage for substantial cost savings for the lab.

After this initial project, with mentoring from the Principal Investigator, the computational scientist will oversee and develop algorithms for analyzing ensemble genomics data, single cell genomics data, single cell merFISH and sequential oligopaints imaging data, as well as novel molecular connectomics data. They will develop code and data sharing standards for the lab, and create SOPs for interfacing with GEO and bitbucket.

They will train the laboratory on code backup in bitbucket and computational lab notebooks. Along with the PI, they will co-lead specific complex interdisciplinary neuroscience projects and spearhead algorithm development for crucial papers in the lab. After the building of infrastructure, ample opportunities for creative advances, grant writing, and drafting/editing manuscripts will be available. The individual will be responsible for mentoring at minimum 1-3 junior analysts to ensure all sequencing and imaging data is housed, formatted, and stored on the computational cluster (and their own in-house servers) according to Cremins lab protocols.

Primary

Duties & Responsibilities:

• Collaborates on designing, conducting and reporting of research projects.
• Leads the process of monthly cleaning of servers and network attached storage across a 25 person laboratory to facilitate major long-term cost savings for the lab.
• Oversees the code development and data storage and accessibility needs of the laboratory toward discovery-based science across all genomics, imaging, and connectome data. Develops key algorithm in collaboration with other graduate students, postdocs, and scientists in the laboratory to advance the laboratories papers and discoveries.
• Trains to achieve mastery and excellence in code to call compartments, TADs, subTADs, loops in Hi-C/Micro-C data, as well as in peak calling of Cut&Run and Cut&Tag data. Oversees trainees to ensure code and parameter selection implemented at highest standards.
• Creates algorithms to analyze data generated by their projects to create paper figures and make discoveries, guides lab members to write and edit methods sections and figures for research papers where the analysis is used in the manuscript.
• Assists with grant preparation and reporting (Editing,…