Biological Scientist

Biological Scientist We use cookies on this site with your consent to analyze our traffic and optimize your user experience. For more information on the types of cookies we use and how you can manage cookies, visit our Cookie Policy. By clicking “Accept Cookies” you agree to the storing of cookies on your device. Please note that if you opt-out, you may not be able to use the full functionalities of the website.###

Biological Scientist Apply:
US - MD, Rockville:
Full time:
Posted Today:
45746
** Job Family:
** Scientist
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* Travel Required:

** None
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* Clearance Required:

** Ability to Obtain Public Trust We are looking for a Biological Scientist to support advanced molecular, cellular, genomic, and translational research programs for NIH. This position is responsible for designing, executing, and optimizing laboratory studies utilizing genome engineering, stem cell biology, high-throughput screening, sequencing, imaging, and functional genomics technologies. This position is a full-time position located in Rockville, MD.

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* What You Will Do:

** This position is responsible for designing, executing, and optimizing laboratory studies utilizing genome engineering, stem cell biology, high-throughput screening, sequencing, imaging, and functional genomics technologies.  
* Design, execute, and optimize molecular biology experiments using gene expression analysis and functional genomics; design and generate genetic constructs through molecular cloning, plasmid engineering, CRISPR/Cas genome editing, and viral vector preparation.
* Isolate, quantify, and assess DNA, RNA, and proteins using manual and automated workflows; prepare and process samples, including library preparation and quality control, for PCR, qPCR, NGS, transcriptomics, and single-cell sequencing to generate genomic and transcriptomic data. Coordinate downstream analyses with bioinformatics teams.
* Coordinate cell line quality control, including mycoplasma testing, karyotyping, STR/DNA fingerprinting, contamination monitoring, and genetically modified cell line validation; manage cell banking activities, including cryopreservation, recovery, authentication, distribution, shipment, receipt, and documentation.
* Perform and optimize mammalian cell culture experiments with primary cells, stem cells, immortalized cell lines, and differentiated populations; differentiate stem cells into specialized cell types using established and novel protocols, automated culture systems, and high-throughput platforms.
* Perform protein characterization and cellular analyses using Western blotting, immunocytochemistry, and immunofluorescence staining; prepare and analyze tissue specimens using cryostat sectioning, microscopy, and histological techniques. Perform fluorescence and confocal imaging and quantitative image analysis.
* Develop, validate, and implement fluorescence-, bioluminescence-, and reporter-based high-throughput screening assays; perform cellular functional assays using flow cytometers, Seahorse metabolic flux analyzers, and multi-electrode arrays.
* Collect, analyze, and interpret experimental data; prepare technical reports, manuscripts, scientific publications, and presentations, and support grant applications. Participate in weekly laboratory meetings to present project updates, findings, and research progress.
* Maintain inventories of reagents, antibodies, plasmids, cell lines, consumables, and experimental resources; operate, calibrate, troubleshoot, and maintain laboratory instrumentation and analytical equipment, including fluorescence and confocal imaging systems. Monitor laboratory operations and infrastructure and coordinate maintenance and instrument services.
* Prepare and maintain protocols, SOPs, electronic laboratory…