Research Specialist II; SOM - Children's

Research Specialist II (SOM - All Children's)

We are seeking a Research Specialist II to join the lab at Johns Hopkins All Children’s Hospital in St. Petersburg, Florida. This position works in a research lab focused on cancer immunology, investigating tumor microenvironment and anti‑tumor immune responses using organoid, primary cell culture systems and in vivo preclinical tumor models. The specialist contributes to immunotherapy and translational research studies within a collaborative and dynamic laboratory environment.

• Conduct assigned laboratory projects.
• Utilize specialized knowledge to interpret results, such as reading controls and evaluating outcomes.
• Work independently, using scientific knowledge and lab experience to make judgments and decisions.
• Teach and train learners and new technicians in the laboratory.
• Guide, direct, and facilitate problem‑solving for entry‑level laboratory members on day‑to‑day challenges with sophisticated research assays.
• Make scientific contributions to protocol development, efficiency, and productivity.
• Collaborate in the development of new techniques and instruments.
• Apply published research methods to current experimental work by investigating protocols and discussing them with the PI.
• Scientifically contribute to the preparation of manuscripts and proposals.
• Process analyzed data into figures for scientific publications.
• Order equipment and supplies and interface with vendors.
• Maintain daily records of experiments.
• Organize and track laboratory samples.
• Other duties as assigned.

• Bachelor's Degree in Biology, Chemistry or a related field.
• Two years of laboratory experience performing relevant techniques, including those commonly used in cancer immunology research.
• Additional education may substitute for the required experience, and additional related experience may substitute for the required education beyond a high school diploma, to the extent permitted by the JHU equivalency formula.

• Master's Degree with related graduate research.
• Mouse handling and tumor model work.
• Organoid and primary cell culture.
• RNA isolation and quantitative PCR (qPCR).
• General cell biology assays.
• Immunostaining.
• Western blotting.
• FACS analysis.
• Proficiency in sterile technique and sample handling.
• Graph Pad/Prism/Adobe proficiency.

• Assay Development and Validation – Developing
• Data Management and Analysis – Developing
• Experimental Design and Execution – Developing
• Operations Management – Developing
• Protocol Development and Optimization – Developing
• Scientific Writing – Developing
• Stakeholder Engagement – Developing

Classified

Title:

Research Specialist II. Role/Level/Range: ACRP/03/MB. Starting Salary Range: $41,300 – $72,300 annually (commensurate with experience). Employee group:
Full Time.

Schedule:

Mon‑Fri, 8:30 am–5:00 pm. FLSA Status:
Exempt.

Location:

Florida. Department:
All Children’s. Personnel area:
School of Medicine.