Senior Imaging Applications Scientist

Senior Imaging Applications Scientist

Biohub is a 501(c)(3) nonprofit research organization supported by the Chan Zuckerberg Initiative, dedicated to advancing biological science through AI‑powered biology. Biohub brings together scientists, engineers, and physicians to pursue grand scientific challenges on 10‑ to 15‑year horizons, focusing on disease mechanisms and innovative diagnostics and therapies.

• We pursue large scientific challenges that cannot be addressed in conventional environments.
• We enable investigators to pursue their riskiest and most innovative ideas.
• The technologies developed at Biohub facilitate research by scientists and clinicians at our home institutions and beyond.

• Serve as an expert for advanced light microscopy systems including light‑sheet, super‑resolution (STED, SIM, SMLM), multiphoton, and custom research instruments.
• Lead the implementation, optimization and maintenance of complex optical systems through alignment, calibration and troubleshooting.
• Collaborate with optical engineers to commission new imaging modalities, validate performance, and transition prototypes to robust research tools.

• Provide high‑level consultation across biological disciplines, guiding modality selection, fluorescent probe strategies, sample preparation and acquisition parameters.
• Troubleshoot complex imaging challenges and implement creative solutions.
• Develop customized protocols for novel biological applications.

• Lead the implementation and optimization of FLIM, FRET, FCS, fluorescence anisotropy and time‑resolved techniques.
• Help develop novel imaging approaches combining multiple modalities.
• Support applications of emerging technologies to challenging biological systems.

• Coordinate scheduling and equitable access to shared microscopy resources and efficient budget management.
• Design acquisition workflows including automated multi‑dimensional imaging and adaptive strategies.
• Collaborate with software developers on user interfaces, data management and analysis pipelines, implementing open‑source and commercial tools.
• Provide training in quantitative image analysis from basic processing to machine‑learning approaches.

• Organize hands‑on workshops, seminars and microscopy training courses.
• Create comprehensive documentation, tutorials and best‑practice guides.
• Represent Biohub at select conferences and contribute to community engagement promoting adoption of advanced imaging methods.

• Ph.D. or equivalent in Physics, Chemistry, Biophysics, Bioengineering, Cell Biology or related field.
• 8+ years of progressive hands‑on experience with advanced light microscopy supporting diverse users.
• Deep expertise in optical microscopy fundamentals: design, fluorescence photophysics, laser systems, optical alignment, system characterization.
• Demonstrated mastery of multiple advanced imaging modalities, especially light‑sheet microscopy (SPIM, lattice light‑sheet) and quantitative fluorescence techniques (FLIM, time‑resolved methods).
• Strong knowledge of fluorescent probe chemistry and photophysics.
• Proficiency with image analysis software and programming (Image
  
  J/Fiji, Python, MATLAB, R) and metadata standards (OME‑TIFF, OME‑ZARR) and imaging data management.
• Outstanding communication skills across audiences from undergraduates to senior investigators.
• Demonstrated success in highly interdisciplinary environments bridging physics, engineering, computer science and biology.
• Strong teaching and mentoring abilities.
• Track record of scientific publication contributions.

• Experience managing core facilities or shared research instrumentation.
• Hands‑on experience with super‑resolution (STED, STORM, PALM, SIM), multiphoton imaging or adaptive optics.
• Experience with modern scientific cameras (sCMOS, EMCCD), detectors (PMTs, SPADs, hybrid detectors) and data acquisition electronics.
• Expertise in high‑content screening, automated imaging or image‑based phenotyping.
• Machine learning or deep learning experience for image analysis.
• Proficiency with microscope control software and hardware…