Research Associate in Bioanalytical Chemistry

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The School of Mathematical and Physical Sciences is seeking to appoint a Research Associate to work on a project funded as part of a BBSRC grant under the guidance of Professor Nick Turner at the University of Sheffield exploring the development of microscopy assays for recognition of extracellular vesicles.

You will form part of a growing team looking at pushing the capabilities of molecular imprinting techniques and targeted recognition polymers at the biological interface. Molecularly Imprinted Polymers (MIPs) are simple, elegant biomimetics that incorporate binding sites bearing steric and chemical functionality complementary to a given target. They represent a generic, versatile, scalable, cost‑effective approach to the creation of synthetic molecular receptors and have uses in separation sciences, purification, sensors and catalysis.

This multi‑disciplinary project will explore the capability of nanoscale MIPs to selectively recognise surface proteins on extracellular vesicles – biological entities that are secreted from cells and act as transporters, signallers and biomarkers. This project is in conjunction with Metaguide

X and OXford Nanoimaging and will require engagement with the Central Laser Facility at Harwell.

You will be a highly motivated individual with a background in molecular imprinting, cell biology and microscopy. Expertise in a good range of common bioanalytical and protein handling techniques will be required, and prior experience of some of the following areas will be advantageous: confocal/electron microscopy, molecular modelling, surface chemistry and molecular imprinting.

You will hold a good first degree in biochemistry, chemistry, or similar; and hold, or be about to examine for, a doctoral level qualification (PhD) in a related subject.

• Work across the University's facilities and with collaborators via regular interaction with academic and industrial partners to ensure the progress of work adheres to the programme timescales. Report problems and non‑compliances to the Project Team.
• Work with external collaborators and represent the University and the Turner group at conferences.
• Design, develop and synthesize molecularly imprinted polymer films and nanomaterials selective for specific surface protein targets.
• Perform, using suitable analytical equipment, affinity and selectivity studies on developed MIPs towards optimisation of MIP/EV interactions.
• Perform, using suitable microscopy equipment, binding studies of MIP/EV interactions.
• Develop attachment strategies for MIPs to STORM microscopy slides.
• Perform where required, microscopy assay development.
• Dissemination of the results of the research as the work progresses, in verbal and written form, through attendance at regular project meetings, both internal and external.
• Promote knowledge and technology exchange when required.
• Comply with the University's Health and Safety, Equal Opportunities and Computing policies in the performance of their duties. Take reasonable care of the Health and Safety of themselves and others affected by their acts or omissions at work.
• Make a full and active contribution to the principles of the Sheffield Academic. These include the achievement of excellence in applied teaching and research, and scholarly pursuits to make a genuine difference in the subject area and to the University's achievements as a whole.
• As a member of staff you will be encouraged to make ethical decisions in your role, embedding the University sustainability strategy into your working activities wherever possible.
• Any other…