Research Technician III Job Toronto area,Ontario Canada,Research/Development

Company Description

UHN is Canada’s #1 hospital and the world’s #1 publicly funded hospital. With 10 sites and more than 44,000 TeamUHN members, UHN consists of Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, The Michener Institute of Education and West Park Healthcare Centre. As Canada's top research hospital, the scope of biomedical research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care.

UHN has the largest hospital-based research program in Canada, with major research in neurosciences, cardiology, transplantation, oncology, surgical innovation, infectious diseases, genomic medicine and rehabilitation medicine. UHN is a research hospital affiliated with the University of Toronto.

UHN’s vision is to build A Healthier World and it’s only because of the talented and dedicated people who work here that we are continually bringing that vision closer to reality.

Job Description

Union: Non-Union
Number of Vacancies: 1
New or Replacement Position: New
Site: Princess Margaret Cancer Centre
Department: Research
Reports to: Principal Investigator
Salary Range: $73,069 - $91,347 Per Year
Hours: Hours Per Week
Shifts: Day shifts;
Monday - Friday
Status: Temporary Full-Time (1 year contract)
Closing Date: August 14, 2026

Position Summary:
Research Technician III serves as wet-lab lead for recombinant protein production and ensuring the technical alignment of the protein production team. This is a senior bench-focused role responsible for the daily execution of experiments, material logistics, and data integrity.

Duties

Protein Production Function as the lead for wet-lab insect cell protein production to meet project milestones.

Collect and record experimental data, ensuring accuracy and rigorous quality control assessments on production batches.

Laboratory Management Support global logistics for protein distribution, managing the team’s inventory and coordinating documentation for all incoming and outgoing shipments

Prepare, assemble, and set up equipment, reagents, and supplies for protein production workflows.

Proactively manage the lifecycle of laboratory consumables, identifying procurement needs and stocking requirements before they impact production timelines.

Organize routine equipment checks, CIP procedures, and notify the lab manager of any issues.

Data & Records Enter and maintain accurate data in laboratory databases and shared records.

Prepare and present summaries of production runs and QC and enforce traceability standards for the labeling and storage of biological samples

Serve as the primary point of contact for the documentation and logistics for material transfers

Health & Safety Follow established laboratory safety guidelines at all biological materials, chemicals, and equipment in compliance with safety protocols.

Contribute to maintaining a safe and orderly work environment.

Qualifications

At minimum, completion of a Bachelor’s degree program, or recognized equivalent in health or science related discipline required

At minimum, 6 years of relevant independent, hands-on experience producing recombinant proteins in insect cells5 years experience working in a structural biology laboratory an asset

Experience with secreted and intracellular insect cell protein expression using BEVS and Sf9

At least 5 years experience purifying recombinant proteins (affinity, IEX, SEC…) and QC using Mass Spectrometry, DSF and/or related methods an asset

Experience in high-throughput protein production

Experience with mammalian systems considered a plus

Proven ability to optimize and scale parallel protein expression and purification

Proficiency with automated FPLC systems

Ability to follow established protocols with accuracy and attention to detail.

Strong record-keeping and data entry skills.

Demonstrated commitment to safety practices.

Ability to work collaboratively in a team environment.

Additional Information

Why join UHN?

In addition to working alongside some of the most talented and inspiring healthcare professionals and researchers in the world, UHN offers a wide range of benefits, programs…