Senior Research Associate​/Research Associate – protein purification

** Join Celltheon and Help Develop the Next Generation of Biologics
**** Celltheon Corporation is seeking an experienced Senior Research Associate / Research Associate– Protein Purification
** to join our downstream process development team. This hands-on laboratory position offers the opportunity to develop, optimize, and scale purification processes for recombinant proteins, monoclonal antibodies, bispecific antibodies, Fc-fusion proteins, and other biologics.

Working closely with scientists across molecular biology, cell line development, upstream process development, and analytics, you will support projects from early research through process development.
** Key Responsibilities
*** Develop and optimize protein purification processes using Protein A, ion exchange (CEX/AEX), hydrophobic interaction (HIC), mixed-mode, IMAC, and size exclusion chromatography (SEC).
* Design and execute purification workflows for recombinant proteins and monoclonal antibodies.
* Perform tangential flow filtration (TFF) for protein concentration, buffer exchange, and formulation.
* Operate, maintain, and troubleshoot ÄKTA FPLC systems.
* Perform routine analytical assays including:
* SEC-HPLC
* Protein A HPLC
* CEX-HPLC
* SDS-PAGE
* UV protein concentration measurements
* Endotoxin testing
* Analyze purification data and recommend process improvements.
* Prepare buffers, chromatography columns, reagents, and laboratory solutions.
* Document experiments in electronic laboratory notebooks (ELNs), batch records, and SOPs.
* Collaborate with cross-functional teams to support client and internal development projects.
* Maintain laboratory equipment and follow laboratory safety and quality procedures.
** Qualifications
* ** Bachelor’s / Master’s degree in Biotechnology, Biochemistry, Chemical Engineering, Bioprocess Engineering, or a related life science discipline.
* ** 2-3 years of industry experience
** in protein purification or downstream process development.
* Strong hands-on experience operating ÄKTA FPLC systems.
* Experience with TFF systems is highly desirable.
* Familiarity with analytical HPLC methods, especially SEC and ion exchange chromatography.
* Experience purifying recombinant proteins and monoclonal antibodies.
* Strong troubleshooting, organizational, and documentation skills.
* Ability to work independently and collaboratively in a fast-paced team environment.
** Preferred Qualifications
*** Experience with process development and purification optimization.
* Familiarity with Unicorn software and chromatography data analysis.
* Experience supporting process scale-up and technology transfer.