DNA Analyst

The Forensic DNA Analyst conducts forensic DNA analysis for criminal investigations and human identification ( HID ) casework, from evidence examination through interpretation, statistical evaluation, and reporting, following validated methods, FBI Quality Assurance Standards ( QAS ), ISO / IEC 17025, and applicable legal and regulatory requirements.

The laboratory operates two validated technology groups, STR and Y- STR by capillary electrophoresis and MPS by sequencing. Authorization to perform casework is technology-specific and is granted by the responsible technical authority, the Primary Technical Leader for STR and Y- STR and the MPS Technical Authority for MPS , following completion of the laboratory training program and demonstration of competency. An analyst may hold authorization in one or both.

Authorization is not a level, and holding authorization in one or both technologies does not change the level of the analyst.

Assigned level (I, II, or III ) reflects the complexity of casework the analyst resolves independently and the review authority held. Every analyst, at every level, is responsible for scientific integrity, accuracy, documentation, chain of custody, and compliance with quality and safety requirements.

All analysts report administratively to the Laboratory Director of Operations and technically to the Technical Leader for their authorized technology. All casework, at every level, undergoes technical review before report issuance. Levels differ in the complexity of the casework the analyst resolves independently and in the review authority held.

Casework is considered routine when two conditions are met: the controls meet all established acceptance criteria, and the interpretation is fully addressed by the approved interpretation guidelines. Routine casework includes mixtures where the interpretation is addressed by the guidelines. Casework that does not meet both conditions is non-routine and is resolved at Level II or above, including complex mixtures, low-template and degraded samples, and unexpected or discordant results.

Level I

Independently performs, interprets, and reports routine casework. Refers non-routine casework to a Level II or higher analyst or to the responsible technical authority. Performs administrative review, technical review of worksheets and work forms, and technical review of case files and reports for single-source results, negative results, and direct comparisons including paternity trios. Affects the analytical accuracy and interpretive soundness of routine casework.

All work is performed within the technology authorization and level assigned to the analyst.

• Examine, screen, and process evidentiary items, including presumptive and confirmatory screening, sample selection, cutting, and specialty processing (bone, petrous, teeth).
• Perform DNA extraction, purification, dilution, concentration, quantification, and amplification.
• Perform capillary electrophoresis setup and instrument operation.
• MPS
  -authorized analysts perform library preparation, target enrichment, sequencing runs, and bioinformatic processing.
• Run controls, evaluate quality control results against acceptance criteria, and determine acceptance of the data, including data generated by an authorized DNA Technician.

• Analyze electropherograms and sequencing data across reference, single-source, and questioned samples, partial and mixture.
• Interpret single-source profiles and mixtures.
• Perform forensic statistical calculations, including random match probability and CPI / CPE .
• Perform relationship and kinship calculations, including trio, single-parent, full sibling, and half sibling, by software and by hand.

• Prepare forensic, relationship, and database reports, subject to technical review.
• Perform administrative review and technical review within the authorized scope and level.
• Prepare for and provide court testimony when authorized.

• Maintain chain of custody, sample integrity, and accurate, complete, and defensible case documentation.
• Communicate results and case status to authorized stakeholders, and elevate technical questions to the Technical Leader.
• Prepare reagents and solutions, and perform routine maintenance, equipment checks, and calibration as assigned.
• In the absence of an assigned DNA Technician, perform evidence reception,…