E11 Bio - Scientist​/Senior Scientist, Molecular Engineering & Sample Optimization

E11 Bio is on a mission to advance connectomics towards whole brain scale for humans and other mammals. We are a collaborative and interdisciplinary team of scientists and engineers engaged in a neuroscience moonshot project to develop a radical technology platform for scalable mammalian brain mapping. E11 Bio is a non-profit Convergent Research focused research organization located in Alameda, California, pursuing blue-skies neuroscience research yet operating like a nimble, tight-knit start-up.

Read our technology roadmap.

• Develop and deploy an assay to systematically evaluate and improve the fixation quality and preservation of brain tissue

• Establish and integrate quantitative metrics of molecular readout e.g. protein retention, antigenicity, and labeling quality across tissue preparation conditions

• Work closely with in vivo research technicians to design experiments, evaluate results, and optimize SOPs

• Drive the development of data-analysis pipelines to routinely generate key performance indicators of sample tissue quality

• Generate an in-house pipeline for binder design/discovery, validation, and optimization of epitope-binder pairs in hydrogels to improve the readout of epitope barcode bits and relevant native synaptic proteins

• Develop novel highly multiplexed protein readout strategies for protein barcodes and molecular annotation of synapses and cell types

• Defined key indicators for perfusion quality and deploy an assay to routinely capture these.

• Incorporated protein retention, antigenicity, and specificity as per-stage metrics across the sample generation pipeline.

• Deployed early perfusion or fixed tissue readout metrics that predict the final expanded tissue quality, including both overall preservation and specific markers of cellular health

• Recorded a measurable > 20% improvement in expanded ultra structural image contrast compared to current baseline perfusion & gelation protocol

• First new epitope-binder designs in production

• Developed assay for binder compatibility in expanded tissue, and validated this assay against existing pairs

• Identified and validated a cocktail of binders for identifying most synapses via e.g. synaptic vesicle binding

• Evaluated the first set of designed epitope-binder pairs and synaptic protein-binder pairs and qualified in expanded tissue, 5+ binders exceeding commercial SOTA performance.

• With collaborators, improved the multiplexed readout strategy to allow for routine incorporation into the imaging pipeline and a demonstrated increase in key barcode metrics (e.g. imaging speed or entropy increases)

• Identified and validated multiple cocktails of binders for stratifying excitatory and inhibitory synapses

$100,000 - $191,000 a year

Scientist base salary range: $100K - 165K

Senior Scientist base salary range: $127K - 191K

This position is eligible for a team performance based bonus up to 20% of base salary and a 401k match up to 6% of base salary.

Total potential compensation range: $126K - 240K

E11 Bio, LLC is an Equal Employment Opportunity employer that proudly pursues and hires a diverse workforce. We do not make hiring or employment decisions on the basis of race, color, religion or religious belief, ethnic or national origin, nationality, sex, gender, gender-identity, sexual orientation, disability, age, military or veteran status, or any other basis protected by applicable local, state, or federal laws or prohibited by Company policy.

We strive for a healthy and safe workplace and strictly prohibit harassment of any kind.