Study Support Technician II – Technical (Aquatics

Primary Work Address: 19700 Helix Drive, Ashburn, VA, 20147

The Howard Hughes Medical Institute’s Janelia Research Campus is apioneering research center in Ashburn, Virginia, where scientistspursue fundamental questions in the life sciences. Our integrated teams of biologists, computational scientists, and tool-buildersinnovate research practices and technologies to solve biology’sdeepest mysteries. HHMI launched Janelia in 2006, establishing an intellectually enriching environment for scientists to do creative,collaborative, hands-on work. We share our methods, results, andtools with the scientific community.

Janelia Research Campus is an HHMI research institute built totackle scientific problems beyond what any single lab or companycould sustain. Janelia's next decade centers on two big bets:mapping how a vertebrate brain produces complex behavior, and building “AI-in-the-Loop Discovery,” where scientists and AI work together to analyze data, design experiments, and drive discovery at a new scale.

This position provides specialized technical services within

Janelia's Aquatics program of the Vivarium. These services focus on creating and maintaining genetically modified zebrafish and Danionella colonies in support of Janelia’s research program.

Working under minimal supervision, provides expertise with hands-on technical procedures to enable and streamline research.

• An opportunity to broaden research experience in a collaborative environment supporting Janelia's whole-brainvertebrate neuroscience program.
• A team that believes in continuous learning and cultivates an environment of collaboration.
• Collaboration with research labs and other shared resources,including Molecular Genomics, Viral Tools, Quantitative Genomics,and other Vivarium teams.

• Perform technical procedures to maintain the zebrafish anddanionella colonies, including embryo care, setting up and breaking down crosses, and breeding coordination through establishment of astable transgenic line.
• Perform reproductive techniques, including embryomicroinjections, IVF, recovery of cryopreserved sperm, and femaleova expression.
• Conduct whole animal screening, including fluorescent and/orphenotype screening, dye screening, embryo screening, fin clipping(larval, juvenile, and adult), and sperm collection for PCR.
• Track each stage of the microinjection process and provide clear handoffs to Aquatics staff and researchers as embryosprogress through early development.
• Maintain transgenic colony records on related data systems,including crossing events, genotyping results, screeningobservations, injection outcomes, AI-supported screening data, andlive-feed culture records that support colony nutrition.
• Communicate with researchers on breeding colony status,microinjection progress, and other updates as needed.

• Associate's or Bachelor's degree in life sciences or a related field, or an equivalent combination of education and experience.
• Two years of experience with Aquatic husbandry or laboratoryanimal care, preferably in a research setting; experience withtransgenic colony management is a plus.
• Demonstrated ability to perform moderate technical procedures,such as proficient microscope use, embryo injection, fin clipping,embryo screening, and cross setup/breakdown.
• Experience with reproductive techniques such as microinjection,IVF, or related molecular biology methods is a plus.
• Comfort using database systems for animal recordkeeping.
• Enthusiastic and self-motivated individual, with the ability towork independently with minimal supervision and learn tasksquickly.
• Excellent skills in Microsoft Office.
• Demonstrated ability to work in a collaborative, rapidly changing team environment to accomplish mutual goals.
• Excellent interpersonal and communication skills to dealeffectively with research scientists, laboratory technical staff,administrative staff, management, outside contractors, and vendors.

Remaining in a normal seated or standing position for extended periods of time; reaching and grasping by extending hand(s) orarm(s); dexterity to manipulate objects with fingers, for example using a…