Biologist

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision‑making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).

Axle is seeking a Part-Time B iologist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute on Aging (NIA) located in Baltimore, MD.

• Paid Time Off and Paid Holidays
• 401K match up to 5%
• Educational Benefits for Career Growth
• Employee Referral Bonus
• Flexible Spending Accounts:
  • Healthcare (FSA)
  • Parking Reimbursement Account (PRK)
  • Dependent Care Assistant Program (DCAP)
  • Transportation Reimbursement Account (TRN)

Provide support services to satisfy the overall operational objectives of the National Institute on Aging. The primary objective is to provide services and deliverables through performance of support services.

Certifications & Licenses

Bachelor’s degree in Biology, Life Sciences or a related discipline

Biology

MS office suite

Ability to communicate both orally and in writing.

Work products and documents related to cultivating, maintaining, expanding and freezing ES and iPS cell lines from mouse and human; prepare ES and iPS cells for N-linked glycoprotein labeling and analysis and for flow analyses; characterize new iPS cell lines; investigate state of pluripotency of ES and new iPS cell lines; characterize nuclear proteins and cell surface proteins by western blot, immunoprecipitation, immunochemistry, and PCR.

- Ad-Hoc

Work products and documents related to identifying/recommending resolution of discrepancies; recommend/determine the appropriate data to use, record or report.

- Ad-Hoc

Work products and documents related to creating/maintaining an accurate research tracking system and database for all research activities; keep accurate records of daily experiments and results; prepare reports, spreadsheets and slides for presentation and/or publication; write and edit scientific papers; present ongoing findings at various meetings.

- Ad-Hoc

Work products and documents related to performing routine maintenance on laboratory instruments and equipment; ensure supplies are properly stocked; obtain quotes and prepare purchase requests.

- Ad-Hoc

Clean Equipment
- Ad-Hoc

Inspect Equipment
- Ad-Hoc

Meet with lab members to present updates
- Weekly

• Program and use computers to store, process, and analyze data - 1
• Interpret research or operational data - 2
• Record research or operational data - 3
• Collect and analyze biological data about relationships among and between organisms and their environment - 4
• Monitor and observe experiments, recording production and test data for evaluation by research personnel - 5
• Provides technical experience needed to assist in studying the basic principles of plant and animal life

Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function

Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species

Assists with performing basic laboratory experiments and procedure

Analyze experimental data and interpret results to write reports and summaries of findings

Cultivate, maintain, expand and freeze ES and iPS cell lines from mouse and human

Prepare ES and iPS cells for N-linked glycoprotein labeling and analysis by Mass Spectrometry

Prepare ES and iPS cells for flow analyses, including the labeling of the cells with appropriate antibodies

Generate primary cultures of cells for the generation of new iPS cell lines

Characterize new iPS cell lines using appropriate techniques (morphology, staining, growth traits)

Prepare primary cell cultures suitable for iPS cell formation using standard nucleofection based technique

Perform primary cell cultures of mitotically inactivated feeder layers

Design and execute flow cytometry (FACS) preps, proliferation assay, and cell surface receptor analysis

Investigate state of pluripotency of ES and new iPS cell lines

Characterize nuclear proteins and cell surface proteins by western blot, immunoprecipitation, immunochemistry, and PCR

Design primers as needed for cell characterization

Apply a wide range of conventional, established approaches, methods, techniques and solutions to new situations; identify/recommend resolution of discrepancies; recommend/ determine what data to use, record or report

Prepares, organizes and maintains various lab samples, supplies, and equipment

Analyze laboratory findings

Maintain an accurate…