Scientist, Research Scientist, Medical Technologist & Lab Technician

We are looking for a Scientist to execute and optimize high-stakes cell culture, molecular biology, and long-read sequencing workflows. You will play a pivotal role in therapeutic screening and platform advancement. The ideal candidate is a self-starter who can translate high-level experimental goals into reproducible, robust workflows while making data-driven decisions.

• Execute and optimize cell culture and electroporation workflows, maintaining primary and engineered systems to support therapeutic screening.
• Design and run ddPCR assays as primary readouts, owning everything from primer design to data analysis of knockdown efficiency.
• Prepare high-molecular-weight DNA and intact RNA for Pac Bio Revio HiFi and Iso-Seq workflows.
• Execute SMRTbell library prep, including end repair, adapter ligation, and size selection for optimal Revio platform performance.
• Perform quality control using Qubit, Nano Drop, Tape Station/Femto Pulse, and fragment analysis.
• Partner with internal teams and vendors to resolve issues like low yield or suboptimal read lengths; support the transition to automated liquid-handling systems.
• Establish robust SOPs and support the scale-up of molecular assays.

• B.S. in Molecular Biology, Cell Biology, Bioengineering, or a related field (Advanced degree preferred).
• 2+ years of hands‑on experience with core techniques (nucleic acid extraction, PCR/qPCR/ddPCR, cloning).
• 1+ year of experience with aseptic technique for mammalian tissue culture.
• 1+ year of experience in NGS sample prep, library preparation, and associated quality control.
• Direct experience culturing and differentiating human ES cells and/or iPS cells is required.
• Ability to work independently in a fast‑paced environment and clearly communicate technical blockers and progress.

• Cell culture
• Mammalian tissue culture
• Aseptic technique
• Primary and engineered cell systems
• Human ES cell culture
• iPS cell culture
• Cell differentiation
• Electroporation
• Therapeutic screening assays
• Molecular biology
• Nucleic acid extraction
• High‑molecular‑weight DNA preparation
• RNA isolation
• PCR, qPCR, ddPCR assay design
• Primer design
• Knockdown efficiency analysis
• Cloning
• NGS sample preparation
• Long‑read sequencing workflows
• Pac Bio Revio HiFi sequencing
• Iso‑Seq
• SMRTbell library preparation
• End repair
• Adapter ligation
• Size selection
• Quality control (Qubit, Nano Drop, Tape Station, Femto Pulse, fragment analysis)
• Data‑driven experimental optimization
• SOP development
• Assay scale‑up
• Troubleshooting yield and read‑length issues
• Collaboration with cross‑functional teams and vendors
• Automation and liquid‑handling systems
• Independent experimental execution
• Technical communication