Molecular Biology Associate - CRISPR Cures Technology Core

Position Summary

The SRA assists researchers in the CRISPR Cures Technology Core to establish a next generation sequencing protocol to capture both DNA and RNA information from human cells.

• PCR from genomic DNA or cDNA to amplify target genes
• Prepare next generation sequencing libraries to sequence the amplified genes or gene products
• Extract DNA and RNA from cultured human cells
• Culture human cell lines
• Analyze next generation sequencing data to identify mutations and quantify gene expression

• Demonstrated proficiency in maintaining mammalian tissue culture cells and performing nucleic acid delivery to them using off-the-shelf technologies such as electroporation and lipid-based delivery.
• Robust practical familiarity and proficiency with kit-based isolation of DNA and RNA from mammalian tissue culture cells.
• Robust practical familiarity and proficiency with the end-to-end process of using PCR to amplify specific genomic loci, including primer design and PCR condition optimization, as well as set up of PCR reactions in multiwell format.
• Demonstrated proficiency in using both agarose gel electrophoresis and PAGE to characterize PCR reaction products.
• Bachelor's degree in biology relevant major and/or equivalent experience/training.

• Library preparation for next generation sequencing

For information on the comprehensive benefits package offered by the University, please visit the University of California's Compensation & Benefits website.

The budgeted hourly range that the University reasonably expects to pay for this position is $28.69 (step 1.0) - $34.22 (step 9.0).

• This is a non-exempt, bi-weekly paid position.
• This is a full-time (40 hours/week) Career position eligible for full UC benefits.

• To apply, please submit your resume and cover letter.

• This recruitment has 2 openings.
• This position is governed by the terms and conditions in the agreement for the Research Support Professionals Unit (RX) between the University of California and the University Professional and Technical Employees (UPTE). The current bargaining agreement manual can be found at: (Use the "Apply for this Job" box below)..html
• This is not a visa opportunity. This position does not include sponsorship of a new consular H-1B visa petition that would require payment of the $100,000 supplemental fee.
• This position is not eligible for remote work.

The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law.