Scientist, Applications Sample Preparation

Scientist, Applications Sample Preparation About Glyphic:

At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease. To date, we have raised >$80M from venture partners and non-dilutive grant funding to achieve our vision of next generation proteome sequencing.

We are seeking a technically driven, detail-oriented, and collaborative Scientist to apply and optimize sample preparation workflows across Glyphic’s single-molecule protein sequencing platform (ProSE), as well as complementary DNA sequencing, RNA sequencing, and mass spectrometry-based proteomics workflows. Reporting to the Director of Assay Integration, you will execute and refine established sample preparation protocols across a broad range of biological inputs and generate high-quality data that supports ProSE performance benchmarking and Glyphic’s broader multi-omic strategy, combining ProSE, RNA-seq, DNA-seq, Ribo-seq, and mass spectrometry to identify neoantigens, biomarkers, and drug targets

The ideal candidate is comfortable executing and troubleshooting multi-step protocols across both nucleic acid- and protein-based workflows, rapidly adapting established methods to new sample types, and communicating results clearly across multidisciplinary teams. A track record of hands-on sample prep execution across proteomics and NGS platforms, and comfort operating in high-throughput environments, will be key to making an immediate impact.

This is a full-time, exempt, in-person position with work conducted at our Berkeley, CA location.

Sample Preparation & Assay Execution

• Evaluate and process incoming biological sample inputs, including cultured cells, fixed cells, tissue sections, blood, and Key Opinion Leader (KOL) samples - assessing sample-specific constraints and analytical requirements before entering the workflow.
• Extract and quantify nucleic acids and proteins from these samples, applying established extraction methods across diverse matrices.
• Apply and adapt enrichment, depletion, and dynamic-range management protocols to improve input quality for low-abundance targets and optimize ProSE and NGS sequencing yield.
• Perform core molecular biology reactions - buffer preparation, reagent formulation, enzymatic reactions, gel electrophoresis, PCR/qPCR, hybridization-based assays, and amplification workflows - to prepare samples for downstream analysis.
• Generate sequencing- and analysis-ready outputs: NGS libraries for DNA-seq, RNA-seq, and ribosome profiling across a broad set of platforms, and processed samples for ProSE and mass spectrometry-based proteomic readouts.
• Support the generation and sourcing of standard reference materials using cell culture techniques.

• Test protocol variables - reagent concentrations, incubation times, temperature conditions, wash conditions, sample preparation steps, and workflow timing - to optimize assay performance.
• Generate and analyze high-quality experimental data, evaluating assay performance using metrics such as signal intensity, background, reproducibility, specificity, sensitivity, sample quality, and workflow robustness.
• Analyze and interpret large-scale proteomic and genomic datasets using quantitative and statistical approaches.
• Propose and apply in-process controls and analytical tools to build a comprehensive understanding of the factors driving sequencing efficiency across diverse sample and molecule types.
• Collaborate closely with proteomics, assay development, chemistry and data science teams to design experiments, interpret results, and guide project strategy - including cross-platform efforts (ProSE, RNA-seq, DNA-seq, ribosome profiling, and mass spectrometry).

Required :

• Degree in biochemistry, chemical biology, molecular biology, or an equivalent field; research experience including:
• PhD / MS / BS with 0+ / 2+ / 4+ years of relevant experience
• Hands-…