Research Technician

The positions in this job family will assist scientists in performing scientific research, development and/or clinical tasks of varying complexity. In general, they will assist in the set up, operation and maintenance of laboratory instruments and equipment, monitoring experiments, making observations and calculating and recording results.

Located in Boston and the surrounding communities, Dana‑Farber Cancer Institute is a leader in life‑changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS, and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high‑risk and underserved populations.

We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School‑affiliated hospitals.

• OVERVIEW We are seeking a highly motivated Research Technician to join our scientific research team studying mechanisms of cancer initiation and progression as well as novel therapeutic targets. This role contributes to current laboratory‐based projects using functional genomics, organoids and single cell approaches to understand oncogene cooperation and combination therapies. The candidate will work with a mentor to assist in the generation and cultivation of 3D models from primary human tumors and cell lines and help in the characterization of cell surface marker expression using a variety of immunological assays combined with biochemical and molecular biology techniques.
  
  They will perform extensive cell and tissue culture, basic molecular biology techniques relating to DNA/RNA and protein work (cloning, Western blotting, immunoprecipitation, protein purification, etc…). In addition, the successful candidate will perform modern functional perturbation techniques including lentiviral knockdown and genome editing tools to validate candidate targets. The candidate will additionally assist with overall lab management including inventory maintenance, reagent tracking, common stock reagent preparation and other related organizational tasks as needed.
• Set up, adjust, calibrate, clean, maintain and troubleshoot equipment
• Clean, maintain and prepare supplies and work areas and may be responsible for ordering of supplies. Collaborates with Purchasing and may be responsible for negotiating with vendors
• Conduct standardized biological, microbiological or biochemical tests and laboratory analyses, including the collection of information and samples from organic substances, such as blood, drugs, and animals
• Isolate, identify and prepare specimens for examination
• Analyze experimental data and interpret results to write reports and summaries of findings, including grant preparation and presentations, and look for opportunities to co‑author publications. May be expected to participate in formal and information presentations of results to staff
• Participate in grant application process and manuscript preparation as required
• Maintains detailed notebook of all work‑related activities
• Monitors laboratory work to ensure compliance with Environmental Health and Safety information as well as the standard operation procedures specific to each laboratory
• Expected to provide intellectual and interactive commitment to position by keeping up with appropriate literature and research publications
• May be responsible for the development, modification and improvement of standard operating procedures
• Uses computers, computer‑interfaced equipment, robotics or high‑technology applications to perform work duties
• May be responsible for designing and executing advanced experiments and setting strategy
• May be responsible for the examination of animals and specimens to detect the presence of disease or other problems
• May be responsible for monitoring laboratory budgets

KNOWLEDGE, SKILLS, AND ABILITIES

REQUIRED:

• Knowledge of information and techniques related to either biology, chemistry, or medicine is preferred
• Must have…